Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; Department of Medicine, Mackay Medical College, New Taipei City, Taiwan; Division of Allergy, Immunology and Rheumatology, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, Taiwan.
Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan; Division of Thoracic Surgery, Taipei Hospital, Ministry of Health and Welfare, New Taipei City, Taiwan.
Mitochondrion. 2019 Jan;44:65-74. doi: 10.1016/j.mito.2018.01.002. Epub 2018 Jan 11.
We evaluated plasma glutamine levels and basal mitochondrial oxygen consumption rate (mOCR) and basal extracellular acidification rate (ECAR) of peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematous (SLE) patients and healthy controls (HCs). Lower plasma glutamine levels correlated with higher SLE disease activity indexes (p=0.025). Incubated in DMEM containing 100mg/dL glucose, SLE-PBMCs displayed lower mOCR (p=0.018) but similar ECAR (p=0.467) to those of HC-PBMCs, and their mOCR got elevated (p<0.001) without altering ECAR (p=0.239) by supplementation with 2 or 4mM glutamine. We conclude that impaired mitochondrial respiration of SLE-PBMCs could be improved by glutamine under euglycemic condition.
我们评估了系统性红斑狼疮（SLE）患者和健康对照者（HC）的外周血单个核细胞（PBMC）的血浆谷氨酰胺水平以及基础线粒体耗氧率（mOCR）和基础细胞外酸化率（ECAR）。较低的血浆谷氨酰胺水平与较高的 SLE 疾病活动指数相关（p=0.025）。在含有 100mg/dL 葡萄糖的 DMEM 中孵育时，SLE-PBMC 的 mOCR 较低（p=0.018），但 ECAR 与 HC-PBMC 相似（p=0.467），补充 2 或 4mM 谷氨酰胺可使它们的 mOCR 升高（p<0.001），而 ECAR 无变化（p=0.239）。我们的结论是，在血糖正常的情况下，谷氨酰胺可改善 SLE-PBMC 的线粒体呼吸功能障碍。
