Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
Fish Shellfish Immunol. 2018 Mar;74:380-385. doi: 10.1016/j.fsi.2018.01.014. Epub 2018 Jan 12.
Giant Mudskipper, Periophthalmodon schlosseri (Pallas, 1770), is euryhaline, amphibious, and air-breathing fish. These fishes live in close association to mangrove forests and often spend over 90% of time out of water, in adjacent mudflats. They have developed morphological and physiological adaptations to satisfy their unique lifestyles. The skin is the primary interface between the body and the environment, and has a central role in host defence. The initiation of immune responses to antigens in the vertebrate skin has often been attributed to epidermal Langerhans'cells (LC) that are dendritic cells (DC), antigen-presenting cells (APC) which reside in the epidermis. Dendritic cells have been characterized morphologically and functionally in the teleost fish tissues such as rainbow trout, salmonids, medaka, African catfish and zebrafish. However, there is no evidence of the presence of DCs and their role in mudskippers immunity. The aim of this preliminary study was to characterize, through use of specific antibodies: Toll-like receptor 2, S100, serotonin (5-HT), and Vesicular acetylcholine transporter VAChT, a specific DC-like subpopulation in Pn. schlosseri's epidermis.
沙塘鳢（Periophthalmodon schlosseri）是一种广盐性、两栖、能呼吸空气的鱼类。这些鱼与红树林密切相关，经常有超过 90%的时间不在水中，而是在相邻的泥滩上。它们已经发展出了形态和生理上的适应性，以满足其独特的生活方式。皮肤是身体和环境之间的主要界面，在宿主防御中起着核心作用。脊椎动物皮肤中对抗原的免疫反应的启动通常归因于表皮中的树突状细胞（LC），即存在于表皮中的树突状细胞（DC），抗原呈递细胞（APC）。在虹鳟鱼、鲑鱼、斑马鱼等硬骨鱼组织中，已经对树突状细胞进行了形态学和功能上的描述。然而，目前还没有证据表明 DC 及其在泥鳢免疫中的作用。本初步研究的目的是通过使用特定的抗体来描述：Toll 样受体 2、S100、血清素（5-HT）和囊泡乙酰胆碱转运体 VAChT，在 Pn. schlosseri 的表皮中存在一种特定的 DC 样亚群。
