Horak Martin, Zlamal Filip, Iliev Robert, Kucera Jan, Cacek Jan, Svobodova Lenka, Hlavonova Zuzana, Kalina Tomas, Slaby Ondrej, Bienertova-Vasku Julie
Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, Czech Republic.
Research Centre for Toxic Compounds in the Environment, Masaryk University, Kamenice 5, Brno, Czech Republic.
PLoS One. 2018 Jan 16;13(1):e0191060. doi: 10.1371/journal.pone.0191060. eCollection 2018.
The aim of the study was to compare selected extracellular miRNA levels (miR-16, miR-21, miR-93 and miR-222 with the response to 8-week-long explosive strength training (EXPL), hypertrophic strength training (HYP) and high-intensity interval training (HIIT).
30 young male athletes of white European origin (mean age: 22.5 ± 4.06 years) recruited at the Faculty of Sports Studies of Masaryk University were enrolled in this study. The study participants were randomly assigned to three possible training scenarios: EXPL, HYP or HITT and participated in 8-week-long program in given arm. Blood plasma samples were collected at the baseline and at week 5 and 8 and anthropometric and physical activity parameters were measured. Pre- and post-intervention characteristics were compared and participants were further evaluated as responders (RES) or non-responders (NRES). RES/NRES status was established for the following characteristics: 300°/s right leg extension (t300), 60°/s right leg extension (t60), isometric extension (IE), vertical jump, isometric extension of the right leg and body fat percentage (BFP).
No differences in miRNA levels were apparent between the intervention groups at baseline. No statistically significant prediction role was observed using crude univariate stepwise regression model analysis where RES/NRES status for t300, t60, IE, vertical jump and pFM was used as a dependent variable and miR-21, miR-222, miR-16 and miR-93 levels at baseline were used as independent variables. The baseline levels of miR-93 expressed an independent prediction role for responder status based on isometric extension of the right leg (beta estimate 0.76, 95% CI: -0.01; 1.53, p = 0.052).
The results of the study indicate that 8-week-long explosive strength training, hypertrophic strength training and high-intensity interval training regimens are associated with significant changes in miR-16, mir-21, miR-222 and miR-93 levels compared to a baseline in athletic young men.
本研究旨在比较特定细胞外微小RNA水平（miR-16、miR-21、miR-93和miR-222）与为期8周的爆发力训练（EXPL）、增肌力量训练（HYP）和高强度间歇训练（HIIT）的反应之间的关系。
招募了30名来自欧洲白种人的年轻男性运动员（平均年龄：22.5±4.06岁），他们均就读于马萨里克大学体育学院。研究参与者被随机分配到三种可能的训练方案：EXPL、HYP或HITT，并在指定手臂上参加为期8周的训练计划。在基线、第5周和第8周采集血浆样本，并测量人体测量和身体活动参数。比较干预前后的特征，并将参与者进一步评估为反应者（RES）或无反应者（NRES）。针对以下特征确定RES/NRES状态：300°/秒右腿伸展（t300）、60°/秒右腿伸展（t60）、等长伸展（IE）、垂直跳跃、右腿等长伸展和体脂百分比（BFP）。
在基线时，各干预组之间的微小RNA水平没有明显差异。在使用粗单变量逐步回归模型分析时，未观察到统计学上显著的预测作用，其中将t300、t60、IE、垂直跳跃和pFM的RES/NRES状态作为因变量，将基线时的miR-21、miR-222、miR-16和miR-93水平作为自变量。基于右腿等长伸展，miR-93的基线水平对反应者状态表现出独立的预测作用（β估计值0.76，95%CI：-0.01；1.53，p = 0.052）。
研究结果表明，与运动型年轻男性的基线相比，为期8周的爆发力训练、增肌力量训练和高强度间歇训练方案与miR-16、mir-21、miR-222和miR-93水平的显著变化相关。
