Yan Xincheng, Zhu Liying, Yu Yadong, Xu Qing, Huang He, Jiang Ling
College of Food Science and Light Industry, ‡College of Biotechnology and Pharmaceutical Engineering, §College of Chemical and Molecular Engineering, and ⊥College of Pharmaceutical Sciences, Nanjing Tech University , Nanjing 210009, China.
J Agric Food Chem. 2018 Feb 14;66(6):1444-1451. doi: 10.1021/acs.jafc.7b06031. Epub 2018 Feb 1.
We developed an in-situ biocatalytic process that couples autoinduction expression of trehalose synthase (TreS) and whole-cell catalysis for trehalose production. With lactose as the autoinducer, the activity of recombinant TreS in recombinant Escherichia coli was optimized through a visualization method, which resulted in a maximum value of 12 033 ± 730 U/mL in pH-stat fed-batch fermentation mode. Meanwhile, the permeability of the autoinduced E. coli increased significantly, which makes it possible to be directly used as a whole-cell biocatalyst for trehalose production, whereby the byproduct glucose can also act as an extra carbon source. In this case, the final yield of trehalose was improved to 90.5 ± 5.7% and remained as high as 83.2 ± 5.0% at the 10th batch, which is the highest value achieved using recombinant TreS. Finally, an integrated strategy for trehalose production was established, and its advantages compared to the traditional mode have been summarized.
我们开发了一种原位生物催化工艺，该工艺将海藻糖合酶（TreS）的自诱导表达与全细胞催化相结合用于海藻糖生产。以乳糖作为自诱导剂，通过可视化方法优化了重组大肠杆菌中重组TreS的活性，在pH-stat补料分批发酵模式下其最大值达到12033±730 U/mL。同时，自诱导大肠杆菌的通透性显著增加，这使其有可能直接用作生产海藻糖的全细胞生物催化剂，副产物葡萄糖也可作为额外的碳源。在此情况下，海藻糖的最终产量提高到90.5±5.7%，在第10批时仍高达83.2±5.0%，这是使用重组TreS所达到的最高值。最后，建立了一种海藻糖生产的集成策略，并总结了其与传统模式相比的优势。
