Enzyme Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Paholyothin Rd., Klong Luang District, Pathumthani 12120, Thailand.
J Microbiol Biotechnol. 2022 Aug 28;32(8):1054-1063. doi: 10.4014/jmb.2202.02028. Epub 2022 May 20.
Trehalose is a non-conventional sugar with potent applications in the food, healthcare and biopharma industries. In this study, trehalose was synthesized from maltose using whole-cell TBRC 1196 producing trehalose synthase (TreS) as the biocatalyst. The reaction condition was optimized using 1% Triton X-100 permeabilized cells. According to our central composite design (CCD) experiment, the optimal process was achieved at 35°C and pH 8.0 for 24 h, resulting in the maximum trehalose yield of 51.60 g/g after 12 h using an initial cell loading of 94 g/l. Scale-up production in a lab-scale bioreactor led to the final trehalose concentration of 51.91 g/l with a yield of 51.60 g/g and productivity of 4.37 g/l/h together with 8.24 g/l glucose as a byproduct. A one-pot process integrating trehalose production and byproduct bioremoval showed 53.35% trehalose yield from 107.4 g/l after 15 h by permeabilized cells. The residual maltose and glucose were subsequently removed by TBRC 12153, resulting in trehalose recovery of 99.23% with 24.85 g/l ethanol obtained as a co-product. The present work provides an integrated alternative process for trehalose production from maltose syrup in bio-industry.
海藻糖是一种非传统的糖，在食品、医疗保健和生物制药行业有广泛的应用。在这项研究中，我们使用产海藻糖合酶（TreS）的全细胞 TBRC 1196 作为生物催化剂，从麦芽糖合成海藻糖。使用 1% Triton X-100 透性化细胞优化反应条件。根据我们的中心组合设计（CCD）实验，最佳工艺条件为 35°C 和 pH 8.0，24 h 后，初始细胞浓度为 94 g/L，12 h 后海藻糖的最大产量为 51.60 g/g。在实验室规模的生物反应器中放大生产，最终海藻糖浓度为 51.91 g/L，得率为 51.60 g/g，产率为 4.37 g/L/h，同时产生 8.24 g/L 的葡萄糖作为副产物。一锅法工艺集成了海藻糖生产和副产物的生物去除，使用透性化细胞 15 h 后从 107.4 g/L 的麦芽糖糖浆中获得 53.35%的海藻糖得率。然后通过 TBRC 12153 去除剩余的麦芽糖和葡萄糖，海藻糖的回收率为 99.23%，同时获得 24.85 g/L 的乙醇作为副产物。本工作为生物工业中从麦芽糖糖浆生产海藻糖提供了一种综合的替代工艺。
