International Center for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology, State Forestry Administration, Beijing 100102, People's Republic of China.
Genome. 2018 Mar;61(3):151-156. doi: 10.1139/gen-2017-0189. Epub 2018 Jan 16.
The Dof transcription factor (TF) family belongs to a class of plant-specific TFs and is involved in plant growth, development, and response to abiotic stresses. However, there are only very limited reports on the characterization of Dof TFs in moso bamboo (Phyllostachys edulis). In the present research, PheDof TFs showed specific expression profiles based on RNA-seq data analyses. The co-expression network indicated that PheDof12, PheDof14, and PheDof16 might play vital roles during flower development. Cis-regulatory element analysis of these PheDof genes suggested diverse functions. Expression patterns of 12 selected genes from seven different classes under three abiotic stresses (cold, salt, and drought) are further investigated by quantitative real-time PCR. This work will provide useful information for functional analysis and regulation mechanisms of Dof TFs in moso bamboo.
Dof 转录因子(TF)家族属于一类植物特异性 TF,参与植物生长、发育和对非生物胁迫的响应。然而,仅有非常有限的报道涉及到毛竹(Phyllostachys edulis)Dof TF 的特征描述。在本研究中,根据 RNA-seq 数据分析,PheDof TF 表现出特定的表达谱。共表达网络表明,PheDof12、PheDof14 和 PheDof16 可能在花发育过程中发挥重要作用。对这些 PheDof 基因的顺式调控元件分析表明了它们具有多样化的功能。通过定量实时 PCR 进一步研究了来自 7 个不同类别 12 个选定基因在 3 种非生物胁迫(冷、盐和干旱)下的表达模式。这项工作将为毛竹 Dof TF 的功能分析和调控机制提供有用的信息。