College of Life Science, Northeast Agricultural University, Harbin 150030, China.
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China.
Int J Mol Sci. 2023 Mar 8;24(6):5154. doi: 10.3390/ijms24065154.
Copper Chaperone For Superoxide Dismutase (CCS) genes encode copper chaperone for Superoxide dismutase (SOD) and dramatically affect the activity of SOD through regulating copper delivery from target to SOD. SOD is the effective component of the antioxidant defense system in plant cells to reduce oxidative damage by eliminating Reactive oxygen species (ROS), which are produced during abiotic stress. CCS might play an important role in abiotic stress to eliminate the damage caused by ROS, however, little is known about CCS in soybean in abiotic stress regulation. In this study, 31 gene family members were identified from soybean genome. These genes were classified into 4 subfamilies in the phylogenetic tree. Characteristics of 31 genes including gene structure, chromosomal location, collinearity, conserved domain, protein motif, cis-elements, and tissue expression profiling were systematically analyzed. RT-qPCR was used to analyze the expression of 31 under abiotic stress, and the results showed that 5 genes(, , , and ) were significantly induced by some kind of abiotic stress. The functions of these genes in abiotic stress were tested using yeast expression system and soybean hairy roots. The results showed that / participated in drought stress regulation. Soybean hairy roots expressing / showed improved drought stress tolerance, with increased SOD and other antioxidant enzyme activities. The results of this study provide reference value in-depth study CCS gene family, and important gene resources for the genetic improvement of soybean drought stress tolerance.
铜伴侣超氧化物歧化酶（CCS）基因编码超氧化物歧化酶（SOD）的铜伴侣，并通过调节铜从靶标到 SOD 的传递，显著影响 SOD 的活性。SOD 是植物细胞抗氧化防御系统的有效成分，可通过消除活性氧（ROS）来减少氧化损伤，ROS 是在非生物胁迫下产生的。CCS 可能在消除 ROS 引起的损伤方面在非生物胁迫中发挥重要作用，然而，关于大豆中非生物胁迫调节中的 CCS 知之甚少。在这项研究中，从大豆基因组中鉴定出 31 个基因家族成员。这些基因在系统发育树中分为 4 个亚家族。对 31 个基因的特征，包括基因结构、染色体位置、共线性、保守结构域、蛋白质基序、顺式元件和组织表达谱进行了系统分析。使用 RT-qPCR 分析了 31 个在非生物胁迫下的表达，结果表明，5 个基因（、、、和）被某种非生物胁迫显著诱导。使用酵母表达系统和大豆毛状根测试了这些基因在非生物胁迫中的功能。结果表明，/参与干旱胁迫调节。表达/的大豆毛状根显示出提高的干旱胁迫耐受性，SOD 和其他抗氧化酶活性增加。本研究结果为深入研究 CCS 基因家族提供了参考价值，并为大豆抗旱性遗传改良提供了重要的基因资源。
