1 School of Biomedical Sciences and Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
2 Queensland Eye Institute, South Brisbane, Queensland, Australia.
Cell Transplant. 2017 Nov;26(11):1717-1732. doi: 10.1177/0963689717723638.
When used as scaffolds for cell therapies, biomaterials often present basic handling and logistical problems for scientists and surgeons alike. The quest for an appropriate mounting device for biomaterials is therefore a significant and common problem. In this review, we provide a detailed overview of the factors to consider when choosing an appropriate mounting device including those experienced during cell culture, quality assurance, and surgery. By way of example, we draw upon our combined experience in developing epithelial cell therapies for the treatment of eye diseases. We discuss commercially available options for achieving required goals and provide a detailed analysis of 4 experimental designs developed within our respective laboratories in Australia, the United Kingdom, and Belgium.
当生物材料被用作细胞治疗的支架时，它们常常给科学家和外科医生带来基本的操作和后勤问题。因此，寻找合适的生物材料固定装置是一个重要且常见的问题。在这篇综述中，我们详细概述了选择合适的固定装置时需要考虑的因素，包括细胞培养、质量保证和手术过程中遇到的因素。通过举例的方式，我们借鉴了在开发用于治疗眼部疾病的上皮细胞疗法方面的联合经验。我们讨论了实现预期目标的商业上可用的选择，并对我们在澳大利亚、英国和比利时的各自实验室开发的 4 种实验设计进行了详细分析。
