Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Av Candido Rondon, 400, 13083-875 Campinas, SP, Brazil.
Institut de Biotecnologia i de Biomedicina (IBB), Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain.
Nanomedicine (Lond). 2018 Feb;13(3):255-268. doi: 10.2217/nnm-2017-0294. Epub 2018 Jan 17.
Nanoparticle-cell interactions can promote cell toxicity and stimulate particular behavioral patterns, but cell responses to protein nanomaterials have been poorly studied.
By repositioning oligomerization domains in a simple, modular self-assembling protein platform, we have generated closely related but distinguishable homomeric nanoparticles. Composed by building blocks with modular domains arranged in different order, they share amino acid composition. These materials, once exposed to cultured cells, are differentially internalized in absence of toxicity and trigger distinctive cell adaptive responses, monitored by the emission of tubular filopodia and enhanced drug sensitivity.
The capability to rapidly modulate such cell responses by conventional protein engineering reveals protein nanoparticles as tuneable, versatile and potent cell stressors for cell-targeted conditioning.
纳米颗粒与细胞的相互作用会促进细胞毒性，并刺激特定的行为模式，但人们对蛋白质纳米材料的细胞反应研究甚少。
通过在一个简单的、模块化的自组装蛋白质平台中重新定位寡聚化结构域，我们生成了密切相关但可区分的同型纳米颗粒。这些由具有模块化结构域的构建块组成，其排列顺序不同，它们具有相同的氨基酸组成。这些材料一旦暴露于培养细胞中，在没有毒性的情况下会被不同程度地内化，并通过管状丝状伪足的发射和增强的药物敏感性来触发独特的细胞适应性反应。
通过常规的蛋白质工程快速调节这种细胞反应的能力表明，蛋白质纳米颗粒是一种可调节、多功能和有效的细胞应激源，可用于细胞靶向调节。
