Department of Chemistry, National Institute of Technology, Tiruchirappalli 620015, Tamilnadu, India.
Department of Chemistry, Selvamm College of Technology, Namakkal 637003, Tamilnadu, India.
Eur J Pharm Sci. 2018 Apr 30;116:61-69. doi: 10.1016/j.ejps.2018.01.025. Epub 2018 Jan 12.
The (E)-2-((2-hydrohy-5-methylphenylimino) methyl) phenol ligand was synthesized. The receptor was characterized by IR, H and C NMR and CHN analysis. The ligand exhibits colorimetric and fluorometric sensing of Zn and Mg ions in semi-aqueous medium (DMSO-H2O). The receptor was tested with series of transition metal ions (Cr, Fe, Ni, Co, Cu, Zn) and heavy metal ions (Sn, Pd, Ce, Hg, Cd) and the essential human body elements like Ca, Mg, Na and K ions. The naked eye colorimetric sensing was absorbed only for Zn and Mg. Both ions (ZnCl and MgCl in HO), when added to the colorless solutions of the receptor of about 1 equivalence in incremental additions turn the solution into bright turmeric yellow. All other ions remain inactive, in colorimetric sensing. Further the Zn and Mg ions were probed by absorption and emission spectroscopy through incremental addition of respective metal ions. The in-situ deprotonation of the ligand on both Mg and Zn ions binding was confirmed by H NMR titration studies. The imino nitrogen of the receptor is not coordinated to the metal ions. The Job's plot studies reveal the 1:2 binding ratio of metal ions to the receptor. The high fold fluorescence output on metal ions binding was positively used to sense the Zn and Mg ions, separately and together in HeLa cancer cells through cell imaging.
(E)-2-((2-羟基-5-甲基苯亚氨基)甲基)苯酚配体被合成。该受体通过 IR、H 和 C NMR 以及 CHN 分析进行了表征。该配体在半水介质（DMSO-H2O）中对 Zn 和 Mg 离子表现出比色和荧光传感性能。该受体经过一系列过渡金属离子（Cr、Fe、Ni、Co、Cu、Zn）和重金属离子（Sn、Pd、Ce、Hg、Cd）以及重要的人体元素（Ca、Mg、Na 和 K 离子）的测试。裸眼比色传感仅对 Zn 和 Mg 离子有效。当将约 1 当量的受体无色溶液加入到 HO 中的 ZnCl 和 MgCl 离子中时，两种离子都会使溶液变为亮黄色。所有其他离子在比色传感中均保持不变。进一步通过吸收和发射光谱法通过逐步加入相应的金属离子来探测 Zn 和 Mg 离子。通过 H NMR 滴定研究证实了配体在结合 Mg 和 Zn 离子时的原位去质子化。受体的亚氨基氮不与金属离子配位。Job's 图研究揭示了金属离子与受体的 1:2 结合比。金属离子结合后荧光输出的高倍数可用于通过细胞成像分别和同时在 HeLa 癌细胞中检测 Zn 和 Mg 离子。
