Jahn S, Volk H D, Grunow R
Allerg Immunol (Leipz). 1985;31(3):195-201.
Lectin-activated human peripheral blood lymphocytes were able to form thermostable rosettes with sheep red blood cells (SRBC). Capability to form thermostable rosettes (TSER) after lectin treatment was comparable in time kinetics and dose dependence to cell size distribution changes. Enhancement of TSER formation by human lymphocytes exposed to PHA and Con A, however, occurred before 3H-Thymidine incorporation could been registered. Lectin treatment of lymphocytes from 20 healthy donors for 48 hrs induced 49 +/- 4% (PHA), 33 +/- 5% (Con A), and 20 +/- 2% (PWM) thermostable rosette forming cells, whereas in unstimulated cultures were found 4 +/- 1%. Furthermore, thermostable rosette forming cells were enriched by gradient centrifugation and characterized as activated lymphocytes of T-origin by SE rosetting and binding studies with various monoclonal antibodies. It was shown that preparation of TSER forming cells after lectin activation makes it possible to enrich the fraction of lectin-stimulated T lymphocytes.
凝集素激活的人外周血淋巴细胞能够与绵羊红细胞（SRBC）形成热稳定花环。凝集素处理后形成热稳定花环（TSER）的能力在时间动力学和剂量依赖性方面与细胞大小分布变化相当。然而，暴露于PHA和Con A的人淋巴细胞在3H-胸腺嘧啶核苷掺入可被检测到之前就增强了TSER的形成。用凝集素处理20名健康供体的淋巴细胞48小时，诱导出49±4%（PHA）、33±5%（Con A）和20±2%（PWM）的热稳定花环形成细胞，而在未刺激的培养物中发现为4±1%。此外，通过梯度离心富集热稳定花环形成细胞，并通过SE花环形成和与各种单克隆抗体的结合研究将其表征为T起源的活化淋巴细胞。结果表明，凝集素激活后制备TSER形成细胞能够富集凝集素刺激的T淋巴细胞部分。
