Ishikawa T, Seguchi H
Anat Embryol (Berl). 1985;173(1):7-11. doi: 10.1007/BF00707299.
Mg++-dependent adenosine triphosphatase (Mg-ATPase) and alkaline phosphatase (ALPase) activities were histo- and cytochemically investigated in postimplantation mouse embryos from day 5 to day 6. In day 5 postimplantation embryos, Mg-ATPase activity was detected in the embryonic ectoderm and weakly in the visceral endoderm. Weak ALPase activity was found in the embryonic ectoderm and visceral endoderm. Parietal endoderm, both in day 5 and in day 6 embryos, had very weak or no Mg-ATPase and ALPase activities. Mg-ATPase activity in day 6 embryos was found with the same localization as that in day 5 embryos. No ALPase activity was observed in their embryonic ectoderm. Extraembryonic ectodermal cell mass had the strongest Mg-ATPase activity in these stage embryos. These results suggest that the localization of both enzyme activities in postimplantation mouse embryos is closely related to the morphogenesis. As regards the proamniotic cavity formation, the fact that Mg-ATPase activity was still observed in the embryonic ectoderm in these stages suggests the involvement of active transport system on the production of nascent proamniotic cavity fluid.
对植入后第5天至第6天的小鼠胚胎进行了组织化学和细胞化学研究，检测了镁离子依赖性三磷酸腺苷酶（Mg-ATPase）和碱性磷酸酶（ALPase）的活性。在植入后第5天的胚胎中，Mg-ATPase活性在胚胎外胚层中被检测到，在内脏内胚层中较弱。在胚胎外胚层和内脏内胚层中发现了微弱的ALPase活性。在第5天和第6天的胚胎中，壁内胚层的Mg-ATPase和ALPase活性都非常弱或没有活性。第6天胚胎中的Mg-ATPase活性与第5天胚胎中的定位相同。在它们的胚胎外胚层中未观察到ALPase活性。在这些阶段的胚胎中，胚外外胚层细胞团具有最强的Mg-ATPase活性。这些结果表明，这两种酶活性在植入后小鼠胚胎中的定位与形态发生密切相关。关于羊膜腔的形成，在这些阶段胚胎的胚胎外胚层中仍观察到Mg-ATPase活性这一事实表明，主动转运系统参与了新生羊膜腔液的产生。
