Fox N W, Damjanov I, Knowles B B, Solter D
Dev Biol. 1984 May;103(1):263-6. doi: 10.1016/0012-1606(84)90028-9.
The distribution of the stage-specific embryonic antigen SSEA-3 was studied immunohistochemically on postimplantation mouse embryos. This carbohydrate antigen, identified as an epitope of a globo-series ganglioside isolated from human teratocarcinoma cells (Kannagi et al., 1983, J. Biol. Chem. 258, 8934-8942) was originally detected on the zygote and mouse early cleavage-stage embryos. It disappears on the early blastocyst and reappears on the primitive endoderm of the implanting blastocyst (Shevinsky et al., 1982, Cell 30, 697-705). We now show in the early egg cylinder (on the sixth day of pregnancy) SSEA-3 is present in the entire visceral endoderm but not in any other part of the conceptus. From Day 7 of pregnancy onward, SSEA-3 is restricted to the extraembryonic visceral endoderm and the visceral yolk sac cells. Therefore, SSEA-3 is a useful marker for this endodermal cell lineage in midgestational mouse embryos.
运用免疫组织化学方法，对植入后小鼠胚胎阶段特异性胚胎抗原SSEA - 3的分布情况进行了研究。这种碳水化合物抗原，被鉴定为从人畸胎癌细胞中分离出的球系列神经节苷脂的一个表位（神谷等人，1983年，《生物化学杂志》258卷，8934 - 8942页），最初是在合子和小鼠早期卵裂期胚胎上检测到的。它在早期囊胚期消失，而在植入囊胚的原始内胚层重新出现（舍温斯基等人，1982年，《细胞》30卷，697 - 705页）。我们现在发现，在早期卵柱期（妊娠第6天），SSEA - 3存在于整个脏内胚层，但在孕体的任何其他部位均未发现。从妊娠第7天起，SSEA - 3局限于胚外脏内胚层和脏卵黄囊细胞。因此，SSEA - 3是中期妊娠小鼠胚胎中这种内胚层细胞谱系的一个有用标记。
