Filyushin M A, Slugina M A, Shchennikova A V, Kochieva E Z
Federal State Institution «Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences», Leninsky pr. 33, bldg. 2, Moscow, 119071, Russia.
Department of Biotechnology, Faculty of Biology, Moscow State University, Leninskie Gory 1, bldg. 12, Moscow, 119991, Russia.
Acta Naturae. 2017 Oct-Dec;9(4):101-109.
Evolution of the genes encoding YABBY transcription factors is believed to be one of the key reasons for flat leaf emergence from the radially symmetrical stem and gynoecium diversity. genes determine the identity of the abaxial surface of all aboveground lateral organs in seed plants. In the present study, complete sequences of orthologous genes were identified and characterized in 13 accessions of cultivated and wild tomato species with diverse morphophysiology of leaves, flowers, and fruits. The obtained gene sequences showed high homology (95-99%) and an identical exon-intron structure with the known gene, and they contained sequences that encode the conserved HMG-like YABBY and Cys2Cys2-zinc-finger domains. In total, in the analyzed genes, 317 variable sites were found, wherein 8 of 24 exon-specific SNPs were nonsynonymous. In the vegetative and reproductive organs of red-fruited and green-fruited tomato species, gene expression was similar to that in described earlier, but it demonstrated interspecies differences at the leaf-, bud- and flower-specific expression levels.
编码YABBY转录因子的基因进化被认为是从径向对称的茎和雌蕊多样性中出现扁平叶的关键原因之一。这些基因决定了种子植物中所有地上侧生器官远轴面的特征。在本研究中，在13个具有不同叶、花和果实形态生理特征的栽培和野生番茄品种中鉴定并表征了直系同源基因的完整序列。获得的基因序列与已知基因显示出高度同源性（95-99%）和相同的外显子-内含子结构，并且它们包含编码保守的HMG样YABBY和Cys2Cys2锌指结构域 的序列。总共，在分析的基因中发现了317个可变位点，其中24个外显子特异性SNP中有8个是非同义的。在红果和绿果番茄品种的营养和生殖器官中，基因表达与之前描述的相似，但在叶、芽和花特异性表达水平上表现出种间差异。
