Catani Martina, Felletti Simona, Ismail Omar H, Gasparrini Francesco, Pasti Luisa, Marchetti Nicola, De Luca Chiara, Costa Valentina, Cavazzini Alberto
Department of Chemistry and Pharmaceutical Sciences, University of Ferrara, via L. Borsari 46, 44121, Ferrara, Italy.
Department of Drug Chemistry and Technology, "Sapienza" University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
Anal Bioanal Chem. 2018 Apr;410(10):2457-2465. doi: 10.1007/s00216-017-0842-4. Epub 2018 Jan 16.
About ten years after their introduction to the market (happened in 2006), the so-called second generation superficially porous particles (SPPs) have undoubtedly become the benchmark as well as, very often, the preferred choice for many applications in liquid chromatography (LC), when high efficiency and fast separations are required. This trend has interested practically all kinds of separations, with the only exception of chiral chromatography (at least so far). The technology of production of base SPPs is advanced, relatively simple and widely available. The deep investigation of mass transfer mechanisms under reversed-phase (RP) and normal-phase (NP) conditions for achiral separations has shown the advantages in the use of these particles over their fully porous counterparts. In addition, it has been demonstrated that SPPs are extremely suitable for the preparation of efficient packed beds through slurry packing techniques. However, the research in this field is in continual evolution. In this article, some of the most advanced concepts and modern applications based on the use of SPPs, embracing in particular ultrafast chiral chromatography and the design of SPPs with engineered pore structures or very reduced particle diameter, are revised. We describe modern trends in these fields and focus on those aspect where further innovation and research will be required. Graphical Abstract Word cloud of cutting edge applications of superficially porous particles in liquid chromatography.
在所谓的第二代表面多孔颗粒（SPPs）投放市场大约十年后（于2006年上市），当需要高效和快速分离时，它们无疑已成为液相色谱（LC）中许多应用的基准，而且常常是首选。这种趋势几乎涵盖了所有类型的分离，唯一的例外是手性色谱（至少到目前为止是这样）。基础SPPs的生产技术先进、相对简单且广泛可得。对非手性分离在反相（RP）和正相（NP）条件下传质机制的深入研究表明，与完全多孔的颗粒相比，使用这些颗粒具有优势。此外，已经证明SPPs非常适合通过淤浆填充技术制备高效填充床。然而，该领域的研究仍在不断发展。在本文中，对基于SPPs使用的一些最先进概念和现代应用进行了综述，尤其包括超快速手性色谱以及具有工程化孔结构或非常小粒径的SPPs的设计。我们描述了这些领域的现代趋势，并关注那些需要进一步创新和研究的方面。图摘要 表面多孔颗粒在液相色谱中的前沿应用词云图
