Institute for Global Health and Infectious Diseases, School of Medicine, University of North Carolina, Chapel Hill, North Carolina.
Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina.
Am J Trop Med Hyg. 2018 Jan;98(1):77-82. doi: 10.4269/ajtmh.17-0529.
In pregnancy-associated malaria, infected erythrocytes accumulate in the placenta. It is unclear if in polyclonal infections this results in distinct peripheral and placental parasite populations. We used long amplicon deep sequencing of ID1-DBL2X from 15 matched peripheral and placental samples collected at delivery from a high transmission area to determine genetic homology. Despite substantial sequence variation and detecting 23 haplotypes, the matched pairs mostly contained the same genetic variants, with 11 pairs sharing 100% of their variants, whereas others showed some heterogeneity. Thus, at delivery, peripheral and placental parasites appear to intermix and placental genotypes can be inferred through peripheral sampling.
在妊娠相关疟疾中，感染的红细胞会在胎盘内聚集。在多克隆感染的情况下，这是否会导致外周血和胎盘寄生虫群体存在明显差异，目前尚不清楚。我们利用 ID1-DBL2X 的长扩增子深度测序，对来自高传播地区的 15 对分娩时采集的配对外周血和胎盘样本进行了研究，以确定遗传同源性。尽管存在大量的序列变异，并检测到 23 种单倍型，但配对样本大多包含相同的遗传变异，其中 11 对样本共享 100%的变异，而其他样本则存在一定程度的异质性。因此，在分娩时，外周血和胎盘寄生虫似乎会混合在一起，并且可以通过外周血样本来推断胎盘基因型。
