Tanaka Chihiro, Itoh Takafumi, Iwasaki Yukimoto, Mizuno Nobuyuki, Nasuda Shuhei, Murai Koji
Department of Bioscience and Biotechnology, Fukui Prefectural University.
Graduate School of Agriculture, Kyoto University.
Genes Genet Syst. 2018 Jul 13;93(1):25-29. doi: 10.1266/ggs.17-00041. Epub 2018 Jan 17.
The wheat florigen gene Wheat FLOWERING LOCUS T (WFT, which is identical to VRN3) is an integrator of the vernalization, photoperiod and autonomous pathways in wheat flowering. Many studies have indicated that VERNALIZATION 1 (VRN1) directly or indirectly up-regulates WFT expression in leaves. VRN1 encodes an APETALA1/FRUITFULL-like MADS box transcription factor that is up-regulated by vernalization and aging, leading to promotion of flowering. In this study, the VRN1 protein was expressed as a His-Tag fusion protein in Escherichia coli and used in an electrophoretic mobility shift assay (EMSA). The results from the EMSA indicated that the VRN1 protein directly binds to the CArG-box in the promoter region of WFT, suggesting the direct up-regulation of WFT by VRN1 in the leaves of wheat plants.
小麦成花素基因小麦开花位点T（WFT，与VRN3相同）是小麦开花过程中春化、光周期和自主途径的整合因子。许多研究表明，春化基因1（VRN1）直接或间接上调叶片中WFT的表达。VRN1编码一种APETALA1/FRUITFULL样MADS盒转录因子，该转录因子受春化和衰老上调，从而促进开花。在本研究中，VRN1蛋白在大肠杆菌中作为His标签融合蛋白表达，并用于电泳迁移率变动分析（EMSA）。EMSA结果表明，VRN1蛋白直接结合到WFT启动子区域的CArG盒，表明VRN1在小麦植株叶片中直接上调WFT。
