Purohit Sharad, Li Tiehai, Guan Wanyi, Song Xuezheng, Song Jing, Tian Yanna, Li Lei, Sharma Ashok, Dun Boying, Mysona David, Ghamande Sharad, Rungruang Bunja, Cummings Richard D, Wang Peng George, She Jin-Xiong
Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, GA, 30912, USA.
Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, GA, 30912, USA.
Nat Commun. 2018 Jan 17;9(1):258. doi: 10.1038/s41467-017-02747-y.
Glycan-binding proteins (GBPs) play critical roles in diverse cellular functions such as cell adhesion, signal transduction and immune response. Studies of the interaction between GBPs and glycans have been hampered by the availability of high throughput and high-content technologies. Here we report multiplex glycan bead array (MGBA) that allows simultaneous analyses of 384 samples and up to 500 glycans in a single assay. The specificity, sensitivity and reproducibility of MGBA are evaluated using 39 plant lectins, 13 recombinant anti-glycan antibodies, and mammalian GBPs. We demonstrate the utility of this platform by the analyses of natural anti-glycan IgM and IgG antibodies in 961 human serum samples and the discovery of anti-glycan antibody biomarkers for ovarian cancer. Our data indicate that the MGBA platform is particularly suited for large population-based studies that require the analyses of large numbers of samples and glycans.
聚糖结合蛋白（GBPs）在多种细胞功能中发挥关键作用，如细胞黏附、信号转导和免疫反应。由于缺乏高通量和高内涵技术，GBPs与聚糖之间相互作用的研究受到了阻碍。在此，我们报告了多重聚糖微珠阵列（MGBA），它能够在一次检测中同时分析384个样本和多达500种聚糖。使用39种植物凝集素、13种重组抗聚糖抗体和哺乳动物GBPs对MGBA的特异性、敏感性和可重复性进行了评估。我们通过分析961份人血清样本中的天然抗聚糖IgM和IgG抗体以及发现卵巢癌的抗聚糖抗体生物标志物，证明了该平台的实用性。我们的数据表明，MGBA平台特别适用于需要分析大量样本和聚糖的基于人群的大型研究。
