Sun Yun, Ma Guo-Juan, Hu Xiao-Jie, Yin Xiang-Yun, Peng Yan-Hui
Fourth Department of General Surgery, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China.
Outpatient Department, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China.
Oncol Lett. 2017 Dec;14(6):6511-6518. doi: 10.3892/ol.2017.7102. Epub 2017 Sep 28.
It has been reported that LMO1 gene was associated with progression, metastasis and apoptosis of leukemia, colorectal cancer and lung cancer. However, the association of LMO1 and gastric cancer remains unclear. The aim of this study is to analyze the relation between LMO1 expression and apoptosis of gastric cancer cells and explore the clinical implications of LMO1 in gastric cancer tissues. The results demonstrated that expression levels of LMO1 and Bcl-2 proteins in gastric cancer tissues were higher than those in adjacent tissues, whereas the opposite was detected for Bax expression (P<0.05). LMO1 protein was associated with TNM staging and lymph node metastasis in gastric cancer (P<0.05). The survival rate of the patients with positive LMO1 gastric carcinoma was lower than that with negative LOM1 expression, and LMO1 was as an independent prognostic factor in COX survival analysis (P<0.05). LMO1-siRNA transfected MKN45 cells had a significant decrease in LMO1 expression and the cell viability, despite of an increase in the apoptotic rate (P<0.05). Following LMO1-siRNA transfection, Bcl-2 expression decreased, while the expression of Bax increased (P<0.05). It's concluded that overexpressed LMO1 in gastric cancer could be as one of new markers of poor prognosis.
据报道，LMO1基因与白血病、结直肠癌和肺癌的进展、转移及凋亡相关。然而，LMO1与胃癌的关系仍不明确。本研究旨在分析LMO1表达与胃癌细胞凋亡之间的关系，并探讨LMO1在胃癌组织中的临床意义。结果表明，胃癌组织中LMO1和Bcl-2蛋白的表达水平高于相邻组织，而Bax表达情况则相反（P<0.05）。LMO1蛋白与胃癌的TNM分期及淋巴结转移相关（P<0.05）。LMO1阳性胃癌患者的生存率低于LMO1表达阴性的患者，且在COX生存分析中LMO1是一个独立的预后因素（P<0.05）。LMO1-siRNA转染的MKN45细胞中，LMO1表达及细胞活力显著降低，尽管凋亡率有所升高（P<0.05）。LMO1-siRNA转染后，Bcl-2表达降低，而Bax表达升高（P<0.05）。结论是，胃癌中LMO1的过表达可能是预后不良的新标志物之一。
