Han Li, Zhang Xiaoying, Wang Aimin, Ji Yang, Cao Xuelei, Qin Qiaoji, Yu Tao, Huang Huan, Yin Lei
School of Nursing, Qingdao University, Qingdao, China.
Department of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, China.
Front Oncol. 2020 Feb 21;10:184. doi: 10.3389/fonc.2020.00184. eCollection 2020.
Gastric cancer (GC) is the TOP3 leading cause of human mortality in malignant tumors. Notwithstanding, the association between GC and circRNAs is not clear. The purpose of this research was to determine the association between GC progression and circRNAs. The data of circRNAs was obtained from the Gene Expression Omnibus (GEO) database to identify gene, which differentially expressed circRNAs in GC tissues and paired normal tissues. The expression of circRNAs in cancer tissues and normal tissues were tested, and the target circRNA was verified before and after surgery in the plasma. A circRNA-micro(mi)RNA-mRNA competing endogenous RNAs (ceRNAs) network was established, and GO and KEGG analysis are performed. Five candidate circRNAs were identified through bioinformatics analysis. Hsa_circ_0021087 and hsa_circ_0005051 were both downregulated in GC tissues, cells and plasma by RTq-PCR. Additionally, there was a significant difference in the expression of plasma hsa_circ_0021087 in patients with GC at the preoperative and postoperative stages ( < 0.001). Hsa_circ_0021087 also promoted the proliferation of GC cells . Next, the circRNA-miRNA-mRNA network of hsa_circ_0021087 was predicted, which may be associated with the development of GC by bioinformatics analysis. In summary, the aforementioned dual-circular RNAs may have important implications on the potential, novel and non-invasive diagnostic method for patients with GC.
胃癌（GC）是人类恶性肿瘤中导致死亡的三大主要原因之一。尽管如此，GC与环状RNA（circRNAs）之间的关联尚不清楚。本研究的目的是确定GC进展与circRNAs之间的关联。从基因表达综合数据库（GEO）获取circRNAs数据，以鉴定在GC组织和配对的正常组织中差异表达的circRNAs基因。检测了癌组织和正常组织中circRNAs的表达，并在术前和术后对血浆中的靶circRNA进行了验证。建立了circRNA-微小（mi）RNA-信使核糖核酸（mRNA）竞争性内源RNA（ceRNAs）网络，并进行了基因本体（GO）和京都基因与基因组百科全书（KEGG）分析。通过生物信息学分析鉴定出5个候选circRNAs。通过逆转录定量聚合酶链反应（RTq-PCR）检测发现，hsa_circ_0021087和hsa_circ_0005051在GC组织、细胞和血浆中均下调。此外，GC患者术前和术后血浆中hsa_circ_0021087的表达存在显著差异（<0.001）。hsa_circ_0021087还促进了GC细胞的增殖。接下来，预测了hsa_circ_0021087的circRNA-miRNA-mRNA网络，通过生物信息学分析，该网络可能与GC的发生发展有关。综上所述，上述双环状RNA可能对GC患者潜在的、新型的非侵入性诊断方法具有重要意义。
