Wu Juekun, Meng Lili, Long Meijun, Ruan Ying, Li Xi, Huang Yong, Qiu Wanshou
Department of Thyroid and Breast Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510630, P.R. China.
Department of Gynecology and Obstetrics, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. China.
Oncol Lett. 2017 Dec;14(6):6809-6814. doi: 10.3892/ol.2017.7113. Epub 2017 Oct 2.
Previous studies have demonstrated strong anti-tumor effects of ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F), an extract from , in liver, lung, stomach and anaplastic thyroid cancer cells. However, whether 5F inhibits the growth of breast cancer cells remains unclear. The present study assessed the effect of 5F on breast cancer cells. The breast cancer cell lines MCF-7, MDA-MB-231 and SK-BR-3 were each treated with 0, 5, 10, 20 and 40 µg/ml 5F. Morphological changes in the breast cancer cells were assessed using fluorescence microscopy. The proliferation and apoptosis of the breast cancer cells were also examined using Cell Counting Kit-8 and flow cytometry. The levels of B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X apoptosis regulator (Bax), Bcl-2 antagonist/killer (Bak) 1 and caspase-3 in the breast cancer cells were assessed. The results of the present study demonstrated that 5F inhibited the proliferation of MCF-7, MDA-MB-231 and SK-BR-3 breast cancer cells in a concentration- and time-dependent manner. Treatment with 5F also induced the apoptosis of breast cancer cells. MDA-MB-231, MCF-7, and SK-BR-3 cells exhibited apoptotic rates of 40.13, 60.44, and 70.49%, respectively, following incubation with 5F for 24 h. Furthermore, 5F significantly decreased the expression of Bcl-2 and increased the expression of Bax, Bak, and caspase-3 in a concentration-dependent manner. The results of the present study revealed that the extract 5F inhibited the growth of human breast cancer cells in a time- and concentration-dependent manner, and that 5F induced apoptosis of human breast cancer cells.
先前的研究已证明，从[植物名称未给出]中提取的ent-11α-羟基-15-氧代-贝壳杉-16-烯-19-酸（5F）对肝癌、肺癌、胃癌和间变性甲状腺癌细胞具有强大的抗肿瘤作用。然而，5F是否能抑制乳腺癌细胞的生长仍不清楚。本研究评估了5F对乳腺癌细胞的作用。分别用0、5、10、20和40μg/ml的5F处理乳腺癌细胞系MCF-7、MDA-MB-231和SK-BR-3。使用荧光显微镜评估乳腺癌细胞的形态变化。还使用细胞计数试剂盒-8和流式细胞术检测乳腺癌细胞的增殖和凋亡情况。评估乳腺癌细胞中B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X凋亡调节因子（Bax）、Bcl-2拮抗剂/杀手（Bak）1和半胱天冬酶-3的水平。本研究结果表明，5F以浓度和时间依赖性方式抑制MCF-7、MDA-MB-231和SK-BR-3乳腺癌细胞的增殖。5F处理还诱导了乳腺癌细胞的凋亡。与5F孵育24小时后，MDA-MB-231、MCF-7和SK-BR-3细胞的凋亡率分别为40.13%、60.44%和70.49%。此外，5F以浓度依赖性方式显著降低Bcl-2的表达，并增加Bax、Bak和半胱天冬酶-3的表达。本研究结果显示，提取物5F以时间和浓度依赖性方式抑制人乳腺癌细胞的生长，并且5F诱导人乳腺癌细胞凋亡。
