Lv Donglai, Hu Zongtao, Lu Lin, Lu Husheng, Xu Xiuli
Department of Clinical Oncology, The 105 Hospital of The People's Liberation Army, Hefei, Anhui 230031, P.R. China.
Oncol Lett. 2017 Dec;14(6):6999-7010. doi: 10.3892/ol.2017.7134. Epub 2017 Oct 3.
In previous years, three-dimensional (3D) cell culture technology has become a focus of research in tumor cell biology, using a variety of methods and materials to mimic the microenvironment of cultured tumor cells . These 3D tumor cells have demonstrated numerous different characteristics compared with traditional two-dimensional (2D) culture. 3D cell culture provides a useful platform for further identifying the biological characteristics of tumor cells, particularly in the drug sensitivity area of the key points of translational medicine. It promises to be a bridge between traditional 2D culture and animal experiments, and is of great importance for further research in the field of tumor biology. In the present review, previous 3D cell culture applications, focusing on anti-tumor drug susceptibility testing, are summarized.
近年来，三维（3D）细胞培养技术已成为肿瘤细胞生物学研究的焦点，人们使用各种方法和材料来模拟培养肿瘤细胞的微环境。与传统的二维（2D）培养相比，这些3D肿瘤细胞已表现出许多不同的特性。3D细胞培养为进一步鉴定肿瘤细胞的生物学特性提供了一个有用的平台，特别是在转化医学关键点的药物敏感性领域。它有望成为传统2D培养和动物实验之间的桥梁，对肿瘤生物学领域的进一步研究具有重要意义。在本综述中，总结了以往聚焦于抗肿瘤药物敏感性测试的3D细胞培养应用。
