Wang Yan, Sun Hai-Hong, Sui Ming-Hua, Ma Jun-Jie
Department of Hematology, Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China.
Department of Emergency Intensive Care Unit, Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China.
Oncol Lett. 2017 Dec;14(6):8078-8083. doi: 10.3892/ol.2017.7220. Epub 2017 Oct 19.
Acute promyelocytic leukemia (APL) is a subtype of acute myelocytic leukemia. Previous studies have reported a number of functions and therapeutic roles of microRNAs (miRs) in APL, and have suggested that miR-218 acts as a tumor suppressor in a number of types of human cancer; however, its role in APL remains unclear. In the present study, the expression of miR-218 and its effects on the viability and proliferation of HL-60 cells was investigated. Reverse transcription-quantitative polymerase chain reaction analysis demonstrated that miR-218 was frequently downregulated in APL marrow tissues compared with normal marrow tissues. Overexpression of miR-218 significantly inhibited cell proliferation, arrested the cell cycle in the G/G phase and induced apoptosis. In addition, B-cell-specific Moloney murine leukemia virus integration site 1 (BMI-1) mRNA expression was negatively associated with miR-218 expression; BMI-1 mRNA and protein expression were downregulated following transfection with miR-218 mimic. These results indicate that miR-218 functions as tumor suppressor in APL, and the miR-218/BMI-1 signaling axis may be a potential novel diagnostic marker and therapeutic target for the treatment of APL.
急性早幼粒细胞白血病（APL）是急性髓细胞白血病的一种亚型。先前的研究报道了微小RNA（miR）在APL中的多种功能和治疗作用，并表明miR-218在多种人类癌症中发挥肿瘤抑制作用；然而，其在APL中的作用仍不清楚。在本研究中，研究了miR-218的表达及其对HL-60细胞活力和增殖的影响。逆转录-定量聚合酶链反应分析表明，与正常骨髓组织相比，miR-218在APL骨髓组织中经常下调。miR-218的过表达显著抑制细胞增殖，使细胞周期停滞在G/G期并诱导细胞凋亡。此外，B细胞特异性莫洛尼鼠白血病病毒整合位点1（BMI-1）mRNA表达与miR-218表达呈负相关；用miR-218模拟物转染后，BMI-1 mRNA和蛋白表达下调。这些结果表明，miR-218在APL中发挥肿瘤抑制作用，miR-218/BMI-1信号轴可能是治疗APL的潜在新型诊断标志物和治疗靶点。
