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miR-34c 在戊四氮诱导癫痫大鼠认知功能中的作用。

Role of miR-34c in the cognitive function of epileptic rats induced by pentylenetetrazol.

机构信息

Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

出版信息

Mol Med Rep. 2018 Mar;17(3):4173-4180. doi: 10.3892/mmr.2018.8441. Epub 2018 Jan 17.

DOI:10.3892/mmr.2018.8441
PMID:29344671
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5802187/
Abstract

Studies suggest that microRNA (miR)‑34c may serve a role in cognitive function in rodent and primate groups. A previous study demonstrated an increase in miR‑34c expression in chronic epileptic rats with memory disorders, induced by pentylenetetrazol (PTZ). However, the mechanism underlying the effects of miR‑34c on cognitive function in epileptic rats remains unclear. Therefore, the present study investigated alterations in cognitive function in temporal lobe epileptic rats, induced by repeated injections of PTZ, following treatment with an miR‑34c agomir compared with a scramble group. Increased expression of miR‑34c was observed in the agomir group, in addition to an increased deficit in learning and memory function in the Morris water maze test. Glutamate receptor ionotropic N‑methyl‑D‑aspartate (NMDA) 2B (NR2B), phosphorylated (p)‑reduced nicotinamide‑adenine dinucleotide phosphate‑dependent diflavin oxidoreductase 1 (NR1) and p‑glutamate receptor 1 (GluR1) protein expression was detected in the hippocampus using western blotting. Additionally, the downregulation of NR2B, p‑NR1 and p‑GluR1 in the miR‑34c agomir group demonstrated that miR‑34c may serve a negative role in cognitive function in epileptic seizures, by dysregulating NMDA and α-amino-3-hydroxy-5‑methyl‑4‑isoxazolepropionic acid receptors, which are associated with long‑term potentiation.

摘要

研究表明，微小 RNA（miR）-34c 可能在啮齿动物和灵长类动物的认知功能中发挥作用。先前的一项研究表明，戊四氮（PTZ）诱导的记忆障碍慢性癫痫大鼠中 miR-34c 的表达增加。然而，miR-34c 对癫痫大鼠认知功能的影响的机制尚不清楚。因此，本研究探讨了重复注射 PTZ 诱导颞叶癫痫大鼠认知功能改变，与 scramble 组相比，用 miR-34c agomir 处理后。在 agomir 组中观察到 miR-34c 的表达增加，此外，在 Morris 水迷宫测试中学习和记忆功能的缺陷也增加。Western blot 检测到海马中谷氨酸受体离子型 N-甲基-D-天冬氨酸（NMDA）2B（NR2B）、磷酸化（p）-还原型烟酰胺腺嘌呤二核苷酸磷酸依赖性双黄素氧化还原酶 1（NR1）和 p-谷氨酸受体 1（GluR1）蛋白表达增加。此外，miR-34c agomir 组中 NR2B、p-NR1 和 p-GluR1 的下调表明，miR-34c 通过调节与长时程增强相关的 NMDA 和 α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体，在癫痫发作中的认知功能中可能发挥负性作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb0/5802187/57e2d5c4b550/MMR-17-03-4173-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb0/5802187/95283cc13187/MMR-17-03-4173-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb0/5802187/d209555f2865/MMR-17-03-4173-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb0/5802187/decc9c261517/MMR-17-03-4173-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb0/5802187/81075b80307c/MMR-17-03-4173-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb0/5802187/57e2d5c4b550/MMR-17-03-4173-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb0/5802187/95283cc13187/MMR-17-03-4173-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb0/5802187/d209555f2865/MMR-17-03-4173-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb0/5802187/decc9c261517/MMR-17-03-4173-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb0/5802187/81075b80307c/MMR-17-03-4173-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efb0/5802187/57e2d5c4b550/MMR-17-03-4173-g04.jpg

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