• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

GOLPH2，一个位于 ras 信号下游的基因，促进了胰腺导管腺癌的进展。

GOLPH2, a gene downstream of ras signaling, promotes the progression of pancreatic ductal adenocarcinoma.

机构信息

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350108, P.R. China.

School of Life Science and Technology, Tongji University, Shanghai 200092, P.R. China.

出版信息

Mol Med Rep. 2018 Mar;17(3):4187-4194. doi: 10.3892/mmr.2018.8430. Epub 2018 Jan 15.

DOI:10.3892/mmr.2018.8430
PMID:29344673
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5802189/
Abstract

Various studies have previously demonstrated that Golgi protein-73 (GOLPH2) is overexpressed in tumorigenesis, which has been observed in hepatocellular carcinoma and prostate cancer. However, the expression levels and specific functions of GOLPH2 in the progression of pancreatic cancer remain to be elucidated. The present study aimed to investigate the expression of GOLPH2 in pancreatic ductal adenocarcinoma (PDAC) tissues and examined the effects of GOLPH2 on the growth and migration of pancreatic cancer cells. In the present study, the mRNA levels of GOLPH2 in PDAC cancer tissues were examined using RT‑qPCR. The effects of GOLPH2 on the growth and migration of cancer cells were examined using crystal violet and Boyden chamber assays. The study demonstrated that the expression of GOLPH2 mRNA and protein was elevated in PDAC clinical tissues. The growth and motility of the PDAC cells was enhanced following overexpression of GOLPH2, whereas downregulating the expression of GOLPH2 impaired the growth, motility and tumorigenesis. Furthermore, GOLPH2 was observed to interact with protein kinase B (Akt), which subsequently increased the activity of Akt. In addition, GOLPH2 was revealed as a downstream gene of Ras signaling and promoted the transformation of normal pancreatic cells. The results of the present study revealed the important functions of GOLPH2 in PDAC, and suggest that GOLPH2 may act as a promising therapeutic target for the treatment of PDAC in the future.

摘要

先前的多项研究表明，高尔基糖蛋白 73（GOLPH2）在肿瘤发生中过度表达，在肝癌和前列腺癌中均有观察到。然而，GOLPH2 在胰腺癌进展中的表达水平和具体功能仍有待阐明。本研究旨在探讨 GOLPH2 在胰腺导管腺癌（PDAC）组织中的表达，并研究 GOLPH2 对胰腺癌细胞生长和迁移的影响。在本研究中，使用 RT-qPCR 检测 PDAC 癌组织中 GOLPH2 的 mRNA 水平。使用结晶紫和 Boyden 室测定法检测 GOLPH2 对癌细胞生长和迁移的影响。研究表明，GOLPH2 mRNA 和蛋白的表达在 PDAC 临床组织中上调。过表达 GOLPH2 可增强 PDAC 细胞的生长和迁移能力，而下调 GOLPH2 的表达则会损害细胞的生长、迁移和致瘤能力。此外，还观察到 GOLPH2 与蛋白激酶 B（Akt）相互作用，从而增加了 Akt 的活性。此外，GOLPH2 被揭示为 Ras 信号的下游基因，并促进了正常胰腺细胞的转化。本研究的结果揭示了 GOLPH2 在 PDAC 中的重要功能，并表明 GOLPH2 可能成为未来治疗 PDAC 的有前途的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/dbf35e77ec72/MMR-17-03-4187-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/dd1e7d17fbc1/MMR-17-03-4187-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/a70321405d14/MMR-17-03-4187-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/44a8d072d162/MMR-17-03-4187-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/98bacb21928b/MMR-17-03-4187-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/56eb03073235/MMR-17-03-4187-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/dbf35e77ec72/MMR-17-03-4187-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/dd1e7d17fbc1/MMR-17-03-4187-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/a70321405d14/MMR-17-03-4187-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/44a8d072d162/MMR-17-03-4187-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/98bacb21928b/MMR-17-03-4187-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/56eb03073235/MMR-17-03-4187-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd7b/5802189/dbf35e77ec72/MMR-17-03-4187-g05.jpg

相似文献

1
GOLPH2, a gene downstream of ras signaling, promotes the progression of pancreatic ductal adenocarcinoma.GOLPH2，一个位于 ras 信号下游的基因，促进了胰腺导管腺癌的进展。
Mol Med Rep. 2018 Mar;17(3):4187-4194. doi: 10.3892/mmr.2018.8430. Epub 2018 Jan 15.
2
Overexpression of PD2 leads to increased tumorigenicity and metastasis in pancreatic ductal adenocarcinoma.PD2的过表达导致胰腺导管腺癌的致瘤性和转移增加。
Oncotarget. 2016 Jan 19;7(3):3317-31. doi: 10.18632/oncotarget.6580.
3
Ribonucleoprotein HNRNPA2B1 interacts with and regulates oncogenic KRAS in pancreatic ductal adenocarcinoma cells.核内不均一核糖核蛋白 A2B1 与胰腺导管腺癌细胞中的致癌 KRAS 相互作用并调节其活性。
Gastroenterology. 2014 Oct;147(4):882-892.e8. doi: 10.1053/j.gastro.2014.06.041. Epub 2014 Jul 3.
4
SIRT 1 Overexpression is Associated with Metastasis of Pancreatic Ductal Adenocarcinoma (PDAC) and Promotes Migration and Growth of PDAC Cells.沉默调节蛋白1（SIRT 1）过表达与胰腺导管腺癌（PDAC）转移相关，并促进PDAC细胞的迁移和生长。
Med Sci Monit. 2016 May 12;22:1593-600. doi: 10.12659/msm.896697.
5
Circular RNA circBFAR promotes the progression of pancreatic ductal adenocarcinoma via the miR-34b-5p/MET/Akt axis.环状 RNA circBFAR 通过 miR-34b-5p/MET/Akt 轴促进胰腺导管腺癌的进展。
Mol Cancer. 2020 May 6;19(1):83. doi: 10.1186/s12943-020-01196-4.
6
PKCι Is a Promising Prognosis Biomarker and Therapeutic Target for Pancreatic Cancer.蛋白激酶Cι是一种有前景的胰腺癌预后生物标志物和治疗靶点。
Pathobiology. 2022;89(6):370-381. doi: 10.1159/000521588. Epub 2022 Jul 4.
7
The MAZ transcription factor is a downstream target of the oncoprotein Cyr61/CCN1 and promotes pancreatic cancer cell invasion via CRAF-ERK signaling.MAZ 转录因子是癌蛋白 Cyr61/CCN1 的下游靶点，通过 CRAF-ERK 信号通路促进胰腺癌细胞侵袭。
J Biol Chem. 2018 Mar 23;293(12):4334-4349. doi: 10.1074/jbc.RA117.000333. Epub 2018 Feb 6.
8
LncRNA H19/miR-194/PFTK1 axis modulates the cell proliferation and migration of pancreatic cancer.长链非编码 RNA H19/miR-194/PFTK1 轴调节胰腺癌细胞的增殖和迁移。
J Cell Biochem. 2019 Mar;120(3):3874-3886. doi: 10.1002/jcb.27669. Epub 2018 Nov 26.
9
Nicotine promotes initiation and progression of KRAS-induced pancreatic cancer via Gata6-dependent dedifferentiation of acinar cells in mice.尼古丁通过 Gata6 依赖性去分化胰腺腺泡细胞促进 KRAS 诱导的胰腺癌的发生和进展。
Gastroenterology. 2014 Nov;147(5):1119-33.e4. doi: 10.1053/j.gastro.2014.08.002. Epub 2014 Aug 12.
10
Expression of DRD2 Is Increased in Human Pancreatic Ductal Adenocarcinoma and Inhibitors Slow Tumor Growth in Mice.DRD2 在人胰腺导管腺癌中表达增加，抑制剂可减缓小鼠肿瘤生长。
Gastroenterology. 2016 Dec;151(6):1218-1231. doi: 10.1053/j.gastro.2016.08.040. Epub 2016 Aug 28.

引用本文的文献

1
GOLM1 dictates acquired Lenvatinib resistance by a GOLM1-CSN5 positive feedback loop upon EGFR signaling activation in hepatocellular carcinoma.GOLM1 通过 EGFR 信号激活在肝细胞癌中形成 GOLM1-CSN5 正反馈环来决定获得性仑伐替尼耐药性。
Oncogene. 2024 Oct;43(42):3108-3120. doi: 10.1038/s41388-024-03153-7. Epub 2024 Sep 9.
2
Golgi Phosphoprotein 73: The Driver of Epithelial-Mesenchymal Transition in Cancer.高尔基体磷蛋白73：癌症上皮-间质转化的驱动因素
Front Oncol. 2021 Dec 7;11:783860. doi: 10.3389/fonc.2021.783860. eCollection 2021.
3
GOLM1 Drives Colorectal Cancer Metastasis by Regulating Myeloid-derived Suppressor Cells.

本文引用的文献

1
PI3K/AKT/mTOR and sonic hedgehog pathways cooperate together to inhibit human pancreatic cancer stem cell characteristics and tumor growth.PI3K/AKT/mTOR通路与音猬因子通路共同协作，抑制人类胰腺癌干细胞特性及肿瘤生长。
Oncotarget. 2015 Oct 13;6(31):32039-60. doi: 10.18632/oncotarget.5055.
2
Cancer statistics, 2015.癌症统计数据，2015 年。
CA Cancer J Clin. 2015 Jan-Feb;65(1):5-29. doi: 10.3322/caac.21254. Epub 2015 Jan 5.
3
Epithelium-Specific ETS (ESE)-1 upregulated GP73 expression in hepatocellular carcinoma cells.上皮特异性ETS（ESE）-1上调了肝癌细胞中GP73的表达。
GOLM1通过调节髓源性抑制细胞驱动结直肠癌转移。
J Cancer. 2021 Oct 21;12(23):7158-7166. doi: 10.7150/jca.61567. eCollection 2021.
4
Genetic Variability in Molecular Pathways Implicated in Alzheimer's Disease: A Comprehensive Review.阿尔茨海默病相关分子通路中的遗传变异性：综述
Front Aging Neurosci. 2021 Mar 18;13:646901. doi: 10.3389/fnagi.2021.646901. eCollection 2021.
5
Golgi protein 73, hepatocellular carcinoma and other types of cancers.高尔基体蛋白73与肝细胞癌及其他类型癌症
Liver Res. 2020 Dec;4(4):161-167. doi: 10.1016/j.livres.2020.09.003. Epub 2020 Sep 25.
6
GOLM1 upregulates expression of PD-L1 through EGFR/STAT3 pathway in hepatocellular carcinoma.GOLM1通过表皮生长因子受体/信号转导子和转录激活子3（EGFR/STAT3）通路上调肝细胞癌中程序性死亡受体配体1（PD-L1）的表达。
Am J Cancer Res. 2020 Nov 1;10(11):3705-3720. eCollection 2020.
7
Repositioning Lidocaine as an Anticancer Drug: The Role Beyond Anesthesia.将利多卡因重新定位为抗癌药物：超越麻醉的作用。
Front Cell Dev Biol. 2020 Jul 17;8:565. doi: 10.3389/fcell.2020.00565. eCollection 2020.
8
MicroRNA‑98 suppresses cell growth and invasion of retinoblastoma via targeting the IGF1R/k‑Ras/Raf/MEK/ERK signaling pathway.微小 RNA-98 通过靶向 IGF1R/k-Ras/Raf/MEK/ERK 信号通路抑制视网膜母细胞瘤细胞的生长和侵袭。
Int J Oncol. 2019 Mar;54(3):807-820. doi: 10.3892/ijo.2019.4689. Epub 2019 Jan 17.
9
Research progress on GP73 in malignant tumors.GP73在恶性肿瘤中的研究进展
Onco Targets Ther. 2018 Oct 24;11:7417-7421. doi: 10.2147/OTT.S181239. eCollection 2018.
Cell Biosci. 2014 Dec 8;4(1):76. doi: 10.1186/2045-3701-4-76. eCollection 2014.
4
eEF1A2 promotes cell migration, invasion and metastasis in pancreatic cancer by upregulating MMP-9 expression through Akt activation.真核延伸因子 1A2 通过激活 Akt 上调 MMP-9 的表达促进胰腺癌细胞迁移、侵袭和转移。
Clin Exp Metastasis. 2013 Oct;30(7):933-44. doi: 10.1007/s10585-013-9593-6. Epub 2013 Jun 6.
5
Overexpression of GOLPH3 promotes proliferation and tumorigenicity in breast cancer via suppression of the FOXO1 transcription factor.高尔基体磷蛋白 3 通过抑制 FOXO1 转录因子促进乳腺癌的增殖和致瘤性。
Clin Cancer Res. 2012 Aug 1;18(15):4059-69. doi: 10.1158/1078-0432.CCR-11-3156. Epub 2012 Jun 6.
6
[Diagnosis of tumours of the liver and the biliary tract: new tissue and serum markers].[肝脏和胆道肿瘤的诊断：新的组织和血清标志物]
Pathologe. 2011 Nov;32 Suppl 2:304-9. doi: 10.1007/s00292-011-1467-6.
7
Pancreatitis-induced inflammation contributes to pancreatic cancer by inhibiting oncogene-induced senescence.胰腺炎引发的炎症通过抑制致癌基因诱导的衰老促进胰腺癌的发生。
Cancer Cell. 2011 Jun 14;19(6):728-39. doi: 10.1016/j.ccr.2011.05.011.
8
Up-regulated Golgi phosphoprotein 2 (GOLPH2) expression in lung adenocarcinoma tissue.肺腺癌组织中高尔基体磷蛋白 2（GOLPH2）表达上调。
Clin Biochem. 2010 Aug;43(12):983-91. doi: 10.1016/j.clinbiochem.2010.05.010. Epub 2010 May 24.
9
Alpha-fetoprotein and serum golgi phosphoprotein 2 are equally discriminative in detecting early hepatocellular carcinomas.甲胎蛋白和血清高尔基体磷蛋白2在检测早期肝细胞癌方面具有同等的鉴别能力。
Hepatology. 2009 Jul;50(1):326. doi: 10.1002/hep.23053.
10
Mutations and response to epidermal growth factor receptor inhibitors.突变与对表皮生长因子受体抑制剂的反应
Clin Cancer Res. 2009 Feb 15;15(4):1133-9. doi: 10.1158/1078-0432.CCR-08-0905.