Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, China.
Turpan Eremophytes Botanical Garden, Chinese Academy of Sciences, Turpan, 838008, China.
Environ Sci Pollut Res Int. 2018 Feb;25(5):5040-5046. doi: 10.1007/s11356-018-1196-y. Epub 2018 Jan 17.
Identification and use of lithium (Li) accumulator plants is a promising strategy to remediate Li-contaminated soil. Apocynum venetum is reported as a Li accumulator. However, its tolerance to Li salt during germination is still unknown. The primary aim of this study was to investigate the effects of two Li salts on seed germination of A. venetum. At the same concentrations, germination percentages in LiCl solution were higher than that in LiCO solution. At 25 °C, seeds germinated to 4-90% at 0-400 mmol L LiCl and 3-91% at 0-150 mmol L LiCO. Low concentration (0-50 mmol L) of LiCl did not significantly affect germination percentage. The simulated critical value (when germination percentage is 50%) in LiCl solution is 196 mmol L, and 36 mmol L for LiCO. Activity of α-amylase, contents of MDA, soluble sugar, and proline were dramatically affected by Li salts, especially at medium and late germination stages. When compared with control, α-amylase activity of seeds under 25 mmol L LiCl and 10 mmol L LiCO did not show significant difference. Germination percentage and index, radicle length, and physiological parameters indicate A. venetum seeds are highly tolerant to Li salts during germination, especially LiCl.
鉴定和利用锂（Li）积累植物是修复 Li 污染土壤的一种很有前途的策略。罗布麻被报道为 Li 积累植物。然而，其对 Li 盐在萌发过程中的耐受性尚不清楚。本研究的主要目的是研究两种 Li 盐对罗布麻种子萌发的影响。在相同浓度下，LiCl 溶液中的萌发率高于 LiCO 溶液。在 25°C 下，种子在 0-400 mmol L LiCl 和 0-150 mmol L LiCO 下萌发至 4-90%和 3-91%。低浓度（0-50 mmol L）的 LiCl 对萌发率没有显著影响。LiCl 溶液中的模拟临界值（萌发率为 50%时）为 196 mmol L，LiCO 为 36 mmol L。α-淀粉酶活性、MDA 含量、可溶性糖和脯氨酸含量均受到 Li 盐的显著影响，尤其是在中晚期萌发阶段。与对照相比，25 mmol L LiCl 和 10 mmol L LiCO 处理下种子的α-淀粉酶活性没有显著差异。萌发率和指数、胚根长度和生理参数表明，罗布麻种子在萌发过程中对 Li 盐具有很强的耐受性，尤其是 LiCl。
