Lamb S C, Garver J C
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706.
Biotechnol Bioeng. 1980 Oct;22(10):2097-2118. doi: 10.1002/bit.260221009.
A methane-utilizing mixed culture isolated from activated sludge by selective enrichment at 45°C was found to consist of three interacting species: a methaneutilizing bacterium, a citrate-utilizing bacterium, and a methanol-utilizing bacterium. All three species grew well at 45°C. Three different stable mixed cultures were reconstituted by various combinations of these pure cultures. The nutritional requirements and substrate ranges for each pure culture were determined. The nutritional requirements and substrate ranges for each pure culture were determined. The saturation constant for the methane-utilizing bacterium on methane (K CH 4) and for the methanol-utilizing bacterium on methanol (K CH 3OH) were 1.73 × 10 M and 4.51 × 10 M, respectively. The volumetric mass transfer coefficient for methane (K a) was determined to be 65.6 hr .
通过在45°C下进行选择性富集从活性污泥中分离出的一种利用甲烷的混合培养物被发现由三种相互作用的物种组成：一种利用甲烷的细菌、一种利用柠檬酸盐的细菌和一种利用甲醇的细菌。所有这三种物种在45°C下生长良好。通过这些纯培养物的各种组合重构了三种不同的稳定混合培养物。确定了每种纯培养物的营养需求和底物范围。确定了每种纯培养物的营养需求和底物范围。利用甲烷的细菌对甲烷的饱和常数（K CH 4）和利用甲醇的细菌对甲醇的饱和常数（K CH 3OH）分别为1.73×10 M和4.51×10 M。甲烷的体积传质系数（K a）被确定为65.6 hr 。
