Lamb S C, Garver J C
Department of Biochemistry University of Wisconsin, Madison, Wisconsin 53706.
Biotechnol Bioeng. 1980 Oct;22(10):2119-2135. doi: 10.1002/bit.260221010.
A two-member methane-utilizing mixed culture of bacteria, formed by combining two pure cultures isolated from a naturally occurring methane-utilizing mixed culture, was studied in continuous culture. From the nutritional requirements and substrate ranges of the pure cultures, a mechanism for the interspecific interactions occurring in the mixed culture was proposed. Product formation kinetics were determined in continuous culture for each product involved in the proposed mechanism. From this proposed mechanism a mathematical model was derived based on simple material balance equations around a single-stage chemostat. The steady-state predictions of this model were compared to experimental results obtained from continuous-culture experiments with the two-member methane-utilizing mixed culture. Interspecific interactions occurring in two-member methanol-utilizing and three-member methane-utilizing mixed cultures have also been discussed.
对一种由从天然存在的利用甲烷的混合培养物中分离出的两种纯培养物组合而成的双成员利用甲烷的细菌混合培养物进行了连续培养研究。根据纯培养物的营养需求和底物范围，提出了混合培养物中种间相互作用的机制。针对所提出机制中涉及的每种产物，在连续培养中确定了产物形成动力学。基于围绕单级恒化器的简单物料平衡方程，从该提出的机制推导出一个数学模型。将该模型的稳态预测与利用双成员利用甲烷的混合培养物进行连续培养实验获得的实验结果进行了比较。还讨论了双成员利用甲醇的混合培养物和三成员利用甲烷的混合培养物中发生的种间相互作用。
