玩转生命分子。
Playing with the Molecules of Life.
机构信息
Department of Chemistry , College of William & Mary , P.O. Box 8795, Williamsburg , Virginia 23187 , United States.
Department of Chemistry , The Scripps Research Institute , La Jolla , California 92037 , United States.
出版信息
ACS Chem Biol. 2018 Apr 20;13(4):854-870. doi: 10.1021/acschembio.7b00974. Epub 2018 Mar 2.
Abstract
Our understanding of the complex molecular processes of living organisms at the molecular level is growing exponentially. This knowledge, together with a powerful arsenal of tools for manipulating the structures of macromolecules, is allowing chemists to to harness and reprogram the cellular machinery in ways previously unimaged. Here we review one example in which the genetic code itself has been expanded with new building blocks that allow us to probe and manipulate the structures and functions of proteins with unprecedented precision.
摘要
我们对生物体分子水平上复杂分子过程的理解正在呈指数级增长。这些知识，加上一套强大的大分子结构操作工具，使化学家能够以前所未有的方式利用和重新编程细胞机制。在这里，我们回顾了一个例子，其中遗传密码本身已经扩展到新的构建块，使我们能够以前所未有的精度探测和操作蛋白质的结构和功能。
相似文献
1
Playing with the Molecules of Life.玩转生命分子。
ACS Chem Biol. 2018 Apr 20;13(4):854-870. doi: 10.1021/acschembio.7b00974. Epub 2018 Mar 2.
2
Expanding and reprogramming the genetic code.扩展和重编程遗传密码。
Nature. 2017 Oct 4;550(7674):53-60. doi: 10.1038/nature24031.
3
Expanding the genetic code.扩展遗传密码
Annu Rev Biophys Biomol Struct. 2006;35:225-49. doi: 10.1146/annurev.biophys.35.101105.121507.
4
The exploding genetic code.不断扩展的遗传密码
Chembiochem. 2014 Aug 18;15(12):1691-4. doi: 10.1002/cbic.201402362. Epub 2014 Jul 30.
5
[Reprogramming the genetic code using flexizyme].[使用柔性酶对遗传密码进行重编程]
Tanpakushitsu Kakusan Koso. 2007 Oct;52(13 Suppl):1643-8.
6
Recent advances in the optical control of protein function through genetic code expansion.通过遗传密码扩展实现蛋白质功能的光学控制的最新进展。
Curr Opin Chem Biol. 2018 Oct;46:99-107. doi: 10.1016/j.cbpa.2018.07.011. Epub 2018 Jul 26.
7
[Expansion and reprogramming of genetic code by Flexizyme].[通过柔性酶对遗传密码的扩展与重编程]
Tanpakushitsu Kakusan Koso. 2006 Dec;51(16 Suppl):2496-501.
8
Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins.通过递归遗传重组实现的定向蛋白质进化进展：在治疗性蛋白质中的应用
Curr Opin Biotechnol. 2001 Aug;12(4):361-70. doi: 10.1016/s0958-1669(00)00228-7.
9
Cellular and Biophysical Applications of Genetic Code Expansion.遗传密码扩展的细胞和生物物理应用。
Chem Rev. 2024 Jun 12;124(11):7465-7530. doi: 10.1021/acs.chemrev.4c00112. Epub 2024 May 16.
10
[Alternative bases and synthetic life].[替代碱基与合成生命]
Med Sci (Paris). 2018 Feb;34(2):179-182. doi: 10.1051/medsci/20183402017. Epub 2018 Feb 16.
引用本文的文献
1
Genetically Encoded Lysine Analogues with Differential Light Sensitivity for Activation of Protein Function.具有不同光敏感性以激活蛋白质功能的基因编码赖氨酸类似物。
ChemPhotoChem. 2024 Jul;8(7). doi: 10.1002/cptc.202300312. Epub 2024 Feb 27.
2
Cys-tRNAj as a Second Translation Initiator for Priming Proteins with Cysteine in Bacteria.在细菌中，半胱氨酸-tRNAj作为用半胱氨酸引发蛋白质合成的第二种翻译起始因子。
ACS Omega. 2025 Jan 29;10(5):4548-4560. doi: 10.1021/acsomega.4c08326. eCollection 2025 Feb 11.
3
Optogenetics with Atomic Precision─A Comprehensive Review of Optical Control of Protein Function through Genetic Code Expansion.具有原子精度的光遗传学——通过遗传密码扩展对蛋白质功能进行光学控制的全面综述
Chem Rev. 2025 Feb 26;125(4):1663-1717. doi: 10.1021/acs.chemrev.4c00224. Epub 2025 Feb 10.
4
Cell surface protein-protein interaction profiling for biological network analysis and novel target discovery.用于生物网络分析和新靶点发现的细胞表面蛋白质-蛋白质相互作用分析
Life Med. 2024 Aug 29;3(4):lnae031. doi: 10.1093/lifemedi/lnae031. eCollection 2024 Aug.
5
Probing the Signal Transduction Mechanism of the Light-Activated Adenylate Cyclase OaPAC Using Unnatural Amino Acid Mutagenesis.利用非天然氨基酸诱变探究光激活腺苷酸环化酶OaPAC的信号转导机制
ACS Chem Biol. 2025 Feb 21;20(2):369-377. doi: 10.1021/acschembio.4c00627. Epub 2025 Jan 22.
6
Beyond , Pharmaceutical Molecule Production in Cell-Free Systems and the Use of Noncanonical Amino Acids Therein.此外，无细胞系统中的药物分子生产及其在其中使用非天然氨基酸。
Chem Rev. 2025 Feb 12;125(3):1303-1331. doi: 10.1021/acs.chemrev.4c00126. Epub 2025 Jan 22.
7
Design and application of synthetic 17B-HSD13 substrates reveals preserved catalytic activity of protective human variants.合成17β-羟类固醇脱氢酶13（17B-HSD13）底物的设计与应用揭示了人类保护性变体的保留催化活性。
Nat Commun. 2025 Jan 2;16(1):297. doi: 10.1038/s41467-024-54487-5.
8
Genetically Recoding Respiratory Syncytial Virus to Visualize Nucleoprotein Dynamics and Virion Assembly.对呼吸道合胞病毒进行基因重编码以可视化核蛋白动态和病毒体组装。
ACS Infect Dis. 2025 Jan 10;11(1):95-103. doi: 10.1021/acsinfecdis.4c00321. Epub 2025 Jan 1.
9
Cell-Free Gene Expression: Methods and Applications.无细胞基因表达：方法与应用
Chem Rev. 2025 Jan 8;125(1):91-149. doi: 10.1021/acs.chemrev.4c00116. Epub 2024 Dec 19.
10
Engineering Phages to Fight Multidrug-Resistant Bacteria.改造噬菌体以对抗多重耐药细菌。
Chem Rev. 2025 Jan 22;125(2):933-971. doi: 10.1021/acs.chemrev.4c00681. Epub 2024 Dec 16.
本文引用的文献
1
Expanding the Scope of Single- and Double-Noncanonical Amino Acid Mutagenesis in Mammalian Cells Using Orthogonal Polyspecific Leucyl-tRNA Synthetases.利用正交多特异性亮氨酰 - tRNA合成酶扩展哺乳动物细胞中单非标准氨基酸诱变和双非标准氨基酸诱变的范围
Biochemistry. 2018 Jan 30;57(4):441-445. doi: 10.1021/acs.biochem.7b00952. Epub 2017 Nov 15.
2
Defining the current scope and limitations of dual noncanonical amino acid mutagenesis in mammalian cells.界定哺乳动物细胞中双非标准氨基酸诱变的当前范围和局限性。
Chem Sci. 2017 Oct 1;8(10):7211-7217. doi: 10.1039/c7sc02560b. Epub 2017 Aug 29.
3
Continuous directed evolution of aminoacyl-tRNA synthetases.氨酰-tRNA合成酶的连续定向进化
Nat Chem Biol. 2017 Dec;13(12):1253-1260. doi: 10.1038/nchembio.2474. Epub 2017 Oct 16.
4
Recombinant Macrocyclic Lanthipeptides Incorporating Non-Canonical Amino Acids.重组大环类硫肽中包含非天然氨基酸。
J Am Chem Soc. 2017 Aug 30;139(34):11646-11649. doi: 10.1021/jacs.7b04159. Epub 2017 Aug 15.
5
A Chemoselective Rapid Azo-Coupling Reaction (CRACR) for Unclickable Bioconjugation.一种用于不可点击生物缀合的化学选择性快速偶联反应（CRACR）。
J Am Chem Soc. 2017 Aug 30;139(34):11670-11673. doi: 10.1021/jacs.7b05125. Epub 2017 Aug 16.
6
Fluorescent Protein-Based Turn-On Probe through a General Protection-Deprotection Design Strategy.基于荧光蛋白的开启型探针通过通用的保护-去保护设计策略。
ACS Sens. 2017 Jul 28;2(7):961-966. doi: 10.1021/acssensors.7b00223. Epub 2017 Jun 16.
7
Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids.利用氟化非天然氨基酸对绿色荧光蛋白进行荧光调制
Molecules. 2017 Jul 16;22(7):1194. doi: 10.3390/molecules22071194.
8
Genetic Code Expansion in Zebrafish Embryos and Its Application to Optical Control of Cell Signaling.斑马鱼胚胎中的遗传密码扩展及其在细胞信号光学控制中的应用。
J Am Chem Soc. 2017 Jul 12;139(27):9100-9103. doi: 10.1021/jacs.7b02145. Epub 2017 Jun 28.
9
Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria.在细菌中对磷酸酪氨酸及其不可水解类似物进行基因编码。
Nat Chem Biol. 2017 Aug;13(8):845-849. doi: 10.1038/nchembio.2405. Epub 2017 Jun 12.
10
Intein Zymogens: Conditional Assembly and Splicing of Split Inteins via Targeted Proteolysis.内含肽酶原：通过靶向蛋白水解的分裂内含肽的条件组装和剪接。
J Am Chem Soc. 2017 Jun 21;139(24):8074-8077. doi: 10.1021/jacs.7b02618. Epub 2017 Jun 7.
Zotero 插件