1 Draper, Cambridge, MA, USA.
SLAS Technol. 2018 Aug;23(4):352-363. doi: 10.1177/2472630317749944. Epub 2018 Jan 18.
Emerging cell therapies have created new demands for instruments that will increase processing efficiency. Purification of lymphocytes prior to downstream steps of gene transfer currently relies on centrifugal separation, which has drawbacks in output sample purity and process automation. Here, we present an alternative approach to blood cell purification using acoustic forces in plastic microchannels. We provide details regarding the system's ability to purify lymphocytes relative to other blood cell types while maintaining a high overall recovery, testing performance starting from leukapheresis product, buffy coat, and whole blood. Depending on settings, the device achieves for lymphocytes up to 97% purity and up to 68% recovery, and depletes 98% of monocytes while also reducing red cells and platelets. We expect that future scale-up of our system for increased throughput will enable its incorporation in the cell therapy workflow, and that it could ultimately reduce costs and expand access for patients.
新兴的细胞疗法为能够提高处理效率的仪器创造了新的需求。在进行基因转移的下游步骤之前，淋巴细胞的纯化目前依赖于离心分离，但这种方法存在输出样品纯度和过程自动化方面的缺陷。在这里，我们提出了一种使用塑料微通道中的声能来进行血细胞纯化的替代方法。我们详细介绍了该系统相对于其他血细胞类型进行淋巴细胞纯化的能力，同时保持高总体回收率，从白细胞分离术产品、血浆层和全血开始测试性能。根据设置的不同，该设备可使淋巴细胞的纯度达到 97%，回收率达到 68%，同时耗竭 98%的单核细胞，还能减少红细胞和血小板。我们预计，未来我们的系统将进行扩大规模以提高通量，这将使其能够纳入细胞治疗工作流程，并且最终可以降低成本并扩大患者的受益面。
