Silva R, Dow P, Dubay R, Lissandrello C, Holder J, Densmore D, Fiering J
Boston University Department of Electrical and Computer Engineering, 8 Saint Mary's St., Boston, USA.
Biological Design Center, 610 Commonwealth Ave, Boston, USA.
Biomed Microdevices. 2017 Sep;19(3):70. doi: 10.1007/s10544-017-0210-3.
Acoustic manipulation has emerged as a versatile method for microfluidic separation and concentration of particles and cells. Most recent demonstrations of the technology use piezoelectric actuators to excite resonant modes in silicon or glass microchannels. Here, we focus on acoustic manipulation in disposable, plastic microchannels in order to enable a low-cost processing tool for point-of-care diagnostics. Unfortunately, the performance of resonant acoustofluidic devices in plastic is hampered by a lack of a predictive model. In this paper, we build and test a plastic blood-bacteria separation device informed by a design of experiments approach, parametric rapid prototyping, and screening by image-processing. We demonstrate that the new device geometry can separate bacteria from blood while operating at 275% greater flow rate as well as reduce the power requirement by 82%, while maintaining equivalent separation performance and resolution when compared to the previously published plastic acoustofluidic separation device.
声学操控已成为一种用于微流体中颗粒和细胞分离与浓缩的通用方法。该技术的最新演示利用压电致动器激发硅或玻璃微通道中的共振模式。在此，我们专注于一次性塑料微通道中的声学操控，以便为即时诊断提供一种低成本的处理工具。不幸的是，由于缺乏预测模型，塑料中共振声流体装置的性能受到阻碍。在本文中，我们通过实验设计方法、参数化快速成型以及图像处理筛选构建并测试了一种塑料血菌分离装置。我们证明，新的装置几何结构能够在比之前高出275%的流速下从血液中分离细菌，同时将功率需求降低82%，并且与之前发表的塑料声流体分离装置相比，保持了同等的分离性能和分辨率。
