dys-1突变对太空飞行的秀丽隐杆线虫基因表达谱的影响。

Effect of dys-1 mutation on gene expression profile in space-flown Caenorhabditis elegans.

作者信息

Xu Dan, Gao Ying, Guo Lin, Lin Chenggang, Sun Yeqing

机构信息

Institute of Environmental Systems Biology, Dalian Maritime University, Linghai Road 1 Dalian, 116026, People's Republic of China.

Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, People's Republic of China.

出版信息

Muscle Nerve. 2018 Jan 18. doi: 10.1002/mus.26076.

DOI:10.1002/mus.26076

PMID:29346705

Abstract

INTRODUCTION

Dystrophin-like dys-1 gene expression increases in the body wall muscles of Caenorhabditis elegans after spaceflight (SF). Here we used a dys-1(cx18) mutant to analyze the molecular adaptive responses of C. elegans to SF.

METHODS

DNA microarrays were performed to identify differentially expressed genes between wild-type (WT) and dys-1 mutant worms after SF. We performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses, predicted human diseases, and screened out key genes for human muscle diseases with NextBio.

RESULTS

Gene expression was less affected by SF in the dys-1 mutant than in the WT worms. The dys-1 mutation influenced neuromuscular gene expression (neuropeptide genes, muscle-related genes, and dystrophin-related genes) under SF conditions, among which 15 genes were specifically regulated by dys-1. NextBio analysis predicted that cdka-1, lev-11, unc-27, and unc-94 genes might play critical roles in muscle atrophy.

DISCUSSION

dys-1 Potentially regulates the neuromuscular system in space. Muscle Nerve, 2018.

摘要

引言

太空飞行（SF）后，秀丽隐杆线虫体壁肌肉中的肌营养不良蛋白样dys-1基因表达增加。在此，我们使用dys-1（cx18）突变体来分析秀丽隐杆线虫对太空飞行的分子适应性反应。

方法

进行DNA微阵列分析，以鉴定太空飞行后野生型（WT）和dys-1突变体线虫之间差异表达的基因。我们进行了基因本体论和京都基因与基因组百科全书通路分析，预测人类疾病，并使用NextBio筛选出人类肌肉疾病的关键基因。

结果

与野生型线虫相比，dys-1突变体中基因表达受太空飞行的影响较小。在太空飞行条件下，dys-1突变影响神经肌肉基因表达（神经肽基因、肌肉相关基因和肌营养不良蛋白相关基因），其中15个基因受dys-1特异性调控。NextBio分析预测，cdka-1、lev-11、unc-27和unc-94基因可能在肌肉萎缩中起关键作用。