Kirk R G, Lee P
Microsc Acta Suppl. 1978(2):102-12.
From the results presented, it is clear that X-ray microanalysis can be used to determine the K and Na content in single red blood cells. With a simultaneous determination of Fe content and cation content, it is possible to deduce the sequence of events in the maturation of membrane transport function in relation to hemoglobin synthesis. This kind of deduction can only be made from a cell by cell analysis of the elemental contents. In addition, X-ray microanalysis can be used to measure other elements such as phosphate, which would permit a better understanding of the role of phosphate compound in volume regulation and metabolism of single red cells. Kinetic studies of active transport in single red cells are also possible by measuring the uptake of rubidium which is normally absent in the cell. Rubidium behaves like potassium in its transport characteristics in most red cells. The technique described here can be used to study the normal and the pathological state of single red cells and can also be applied to other single cells such as white cells, bacteria, and dispersed cells in tissue culture.
从所呈现的结果来看，很明显X射线微量分析可用于测定单个红细胞中的钾和钠含量。通过同时测定铁含量和阳离子含量，就有可能推断出与血红蛋白合成相关的膜转运功能成熟过程中的事件顺序。这种推断只能通过对细胞元素含量进行逐个细胞分析来得出。此外，X射线微量分析可用于测量其他元素，如磷酸盐，这将有助于更好地理解磷酸盐化合物在单个红细胞体积调节和代谢中的作用。通过测量细胞中通常不存在的铷的摄取量，也可以对单个红细胞中的主动运输进行动力学研究。在大多数红细胞中，铷在其运输特性方面表现得与钾相似。这里描述的技术可用于研究单个红细胞的正常和病理状态，也可应用于其他单个细胞，如白细胞、细菌和组织培养中的分散细胞。
