Feglo Patrick Kwame, Sewurah Miriam
Department of Clinical Microbiology, School of Medical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
BMC Res Notes. 2018 Jan 18;11(1):45. doi: 10.1186/s13104-017-2923-z.
The purpose of this study was to investigate the virulent factors of Vibrio cholerae which caused an unprecedented large cholera outbreak in Ghana in 2014 and progressed into 2015, affected 28,975 people with 243 deaths.
The V. cholerae isolates were identified to be the classical V. cholerae 01 biotype El Tor, serotype Ogawa, responsible for the large cholera outbreak in Ghana. These El Tor strains bear CtxAB and Tcp virulent genes, making the strains highly virulent. The strains also bear SXT transmissible element coding their resistance to antibiotics, causing high proportions of the strains to be multidrug resistant, with resistant proportions of 95, 90 and 75% to trimethoprim/sulfamethoxazole, ampicillin and ceftriaxone respectively. PFGE patterns indicated that the isolates clustered together with the same pattern and showed clusters similar to strains circulating in DR Congo, Cameroun, Ivory Coast and Togo. The strains carried virulence genes which facilitated the disease causation and spread. This is the first time these virulent genes were determined on the Ghanaian Vibrio strains.
本研究旨在调查2014年在加纳引发了前所未有的大规模霍乱疫情并持续至2015年的霍乱弧菌的致病因素，此次疫情感染了28,975人，造成243人死亡。
霍乱弧菌分离株被鉴定为霍乱弧菌01群古典生物型埃尔托生物型、小川血清型，该血清型导致了加纳的大规模霍乱疫情。这些埃尔托菌株携带CtxAB和Tcp致病基因，使菌株具有高致病性。这些菌株还携带SXT可传递元件，编码其对抗生素的抗性，导致高比例的菌株对多种药物耐药，对甲氧苄啶/磺胺甲恶唑、氨苄西林和头孢曲松的耐药比例分别为95%、90%和75%。脉冲场凝胶电泳（PFGE）图谱表明，分离株以相同的图谱聚集在一起，并且显示出与在刚果民主共和国、喀麦隆、科特迪瓦和多哥传播的菌株相似的聚类。这些菌株携带的致病基因促进了疾病的发生和传播。这是首次在加纳霍乱弧菌菌株上确定这些致病基因。
