人参皂苷Rb1对他克莫司诱导的肾近端小管LLC-PK1细胞凋亡的保护作用。

Protective effect of ginsenoside Rb1 against tacrolimus-induced apoptosis in renal proximal tubular LLC-PK1 cells.

作者信息

Lee Dahae, Lee Dong-Soo, Jung Kiwon, Hwang Gwi Seo, Lee Hye Lim, Yamabe Noriko, Lee Hae-Jeong, Eom Dae-Woon, Kim Ki Hyun, Kang Ki Sung

机构信息

School of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea.

Institute of Human-Environment Interface Biology, Biomedical Research Institute, Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea.

出版信息

J Ginseng Res. 2018 Jan;42(1):75-80. doi: 10.1016/j.jgr.2016.12.013. Epub 2017 Jan 10.

DOI:10.1016/j.jgr.2016.12.013

PMID:29348725

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5766699/

Abstract

BACKGROUND

The aim of the present study was to evaluate the potential protective effects of six ginsenosides (Rb1, Rb2, Rc, Rd, Rg1, and Rg3) isolated from against tacrolimus (FK506)-induced apoptosis in renal proximal tubular LLC-PK1 cells.

METHODS

LLC-PK1 cells were treated with FK506 and ginsenosides, and cell viability was measured. Protein expressions of mitogen-activated protein kinases, caspase-3, and kidney injury molecule-1 (KIM-1) were evaluated by Western blotting analyses. The number of apoptotic cells was measured using an image-based cytometric assay.

RESULTS

Reduction in cell viability by 60μM FK506 was ameliorated significantly by cotreatment with ginsenosides Rg1 and Rb1. The phosphorylation of p38, extracellular signal-regulated kinases, and KIM-1, and cleavage of caspase-3, increased markedly in LLC-PK1 cells treated with FK506 and significantly decreased after cotreatment with ginsenoside Rb1. The number of apoptotic cells decreased by 6.0% after cotreatment with ginsenoside Rb1 (10μM and 50μM).

CONCLUSION

The antiapoptotic effects of ginsenoside Rb1 on FK506-induced apoptosis were mediated by the inhibition of mitogen-activated protein kinases and caspase activation.

摘要

背景

本研究旨在评估从[具体来源未给出]分离出的六种人参皂苷（Rb1、Rb2、Rc、Rd、Rg1和Rg3）对他克莫司（FK506）诱导的肾近端小管LLC-PK1细胞凋亡的潜在保护作用。

方法

用FK506和人参皂苷处理LLC-PK1细胞，并测量细胞活力。通过蛋白质印迹分析评估丝裂原活化蛋白激酶、半胱天冬酶-3和肾损伤分子-1（KIM-1）的蛋白表达。使用基于图像的细胞计数法测量凋亡细胞的数量。

结果

与Rg1和Rb1人参皂苷共同处理可显著改善60μM FK506导致的细胞活力降低。在用FK506处理的LLC-PK1细胞中观察到p38、细胞外信号调节激酶和KIM-1的磷酸化以及半胱天冬酶-3的裂解显著增加，而在与Rb1人参皂苷共同处理后显著降低。与Rb1人参皂苷（10μM和50μM）共同处理后，凋亡细胞数量减少了6.0%。

结论

Rb1人参皂苷对FK506诱导的细胞凋亡的抗凋亡作用是通过抑制丝裂原活化蛋白激酶和半胱天冬酶激活介导的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4da/5766699/b267a16a8c22/gr1.jpg

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人参皂苷Rb1对他克莫司诱导的肾近端小管LLC-PK1细胞凋亡的保护作用。

Protective effect of ginsenoside Rb1 against tacrolimus-induced apoptosis in renal proximal tubular LLC-PK1 cells.

作者信息

Lee Dahae, Lee Dong-Soo, Jung Kiwon, Hwang Gwi Seo, Lee Hye Lim, Yamabe Noriko, Lee Hae-Jeong, Eom Dae-Woon, Kim Ki Hyun, Kang Ki Sung

机构信息

School of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea.

Institute of Human-Environment Interface Biology, Biomedical Research Institute, Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea.