Hosseini Azar, Rajabian Arezoo, Forouzanfar Fatemeh, Farzadnia Mahdi, Boroushaki Mohammad Taher
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Avicenna J Phytomed. 2022 Jul-Aug;12(4):439-448. doi: 10.22038/AJP.2022.19703.
The clinical use of tacrolimus is limited due to its side effects. This research investigated the protective activities of pomegranate seed oil (PSO) against TAC toxicity.
The groups are included normal (1 ml of corn oil), TAC (2 mg/kg), and co-treatment of PSO (0.4 and 0.8 ml/kg) and TAC. All administrations were carried out intraperitoneally for 14 days. After the last injection, blood was collected from the heart.
TAC increased creatinine and urea. Increased malondialdehyde, reduced thiol content and superoxide dismutase. The elevation of lactate dehydrogenase, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine kinase-MB and creatinine phosphokinase that confirmed cardiac toxicity. PSO reduced TAC toxicity. PSO decreased TAC-induced pathology injury.
PSO reduced TAC toxicity in renal and heart via scavenging free radicals.
他克莫司因其副作用而临床应用受限。本研究调查了石榴籽油（PSO）对他克莫司毒性的保护作用。
分组包括正常组（1毫升玉米油）、他克莫司组（2毫克/千克）以及石榴籽油（0.4和0.8毫升/千克）与他克莫司联合治疗组。所有给药均通过腹腔注射进行，持续14天。末次注射后，从心脏采集血液。
他克莫司使肌酐和尿素升高。丙二醛增加，硫醇含量和超氧化物歧化酶降低。乳酸脱氢酶、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、肌酸激酶-MB和肌酸磷酸激酶升高，证实存在心脏毒性。石榴籽油降低了他克莫司的毒性。石榴籽油减轻了他克莫司诱导的病理损伤。
石榴籽油通过清除自由基降低了他克莫司对肾脏和心脏的毒性。
