• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

黑参激活Akt信号通路，从而增强成肌细胞分化和肌管生长。

Black ginseng activates Akt signaling, thereby enhancing myoblast differentiation and myotube growth.

作者信息

Lee Soo-Yeon, Go Ga-Yeon, Vuong Tuan Anh, Kim Jee Won, Lee Sullim, Jo Ayoung, An Jun Min, Kim Su-Nam, Seo Dong-Wan, Kim Jin-Seok, Kim Yong Kee, Kang Jong-Sun, Lee Sang-Jin, Bae Gyu-Un

机构信息

Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul, Republic of Korea.

Department of Molecular Cell Biology, Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.

出版信息

J Ginseng Res. 2018 Jan;42(1):116-121. doi: 10.1016/j.jgr.2017.08.009. Epub 2017 Sep 6.

DOI:10.1016/j.jgr.2017.08.009
PMID:29348730
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5766703/
Abstract

BACKGROUND

Black ginseng (BG) has greatly enhanced pharmacological activities relative to white or red ginseng. However, the effect and molecular mechanism of BG on muscle growth has not yet been examined. In this study, we investigated whether BG could regulate myoblast differentiation and myotube hypertrophy.

METHODS

BG-treated C2C12 myoblasts were differentiated, followed by immunoblotting for myogenic regulators, immunostaining for a muscle marker, myosin heavy chain or immunoprecipitation analysis for myogenic transcription factors.

RESULTS

BG treatment of C2C12 cells resulted in the activation of Akt, thereby enhancing heterodimerization of MyoD and E proteins, which in turn promoted muscle-specific gene expression and myoblast differentiation. BG-treated myoblasts formed larger multinucleated myotubes with increased diameter and thickness, accompanied by enhanced Akt/mTOR/p70S6K activation. Furthermore, the BG treatment of human rhabdomyosarcoma cells restored myogenic differentiation.

CONCLUSION

BG enhances myoblast differentiation and myotube hypertrophy by activating Akt/mTOR/p70S6k axis. Thus, our study demonstrates that BG has promising potential to treat or prevent muscle loss related to aging or other pathological conditions, such as diabetes.

摘要

背景

与白参或红参相比，黑参具有显著增强的药理活性。然而，黑参对肌肉生长的影响及其分子机制尚未得到研究。在本研究中，我们调查了黑参是否能够调节成肌细胞分化和肌管肥大。

方法

对经黑参处理的C2C12成肌细胞进行分化，随后对生肌调节因子进行免疫印迹分析、对肌肉标志物肌球蛋白重链进行免疫染色，或对生肌转录因子进行免疫沉淀分析。

结果

用黑参处理C2C12细胞导致Akt激活，从而增强MyoD和E蛋白的异二聚化，进而促进肌肉特异性基因表达和成肌细胞分化。经黑参处理的成肌细胞形成了更大的多核肌管，其直径和厚度增加，同时Akt/mTOR/p70S6K激活增强。此外，用黑参处理人横纹肌肉瘤细胞可恢复生肌分化。

结论

黑参通过激活Akt/mTOR/p70S6k轴增强成肌细胞分化和肌管肥大。因此，我们的研究表明，黑参在治疗或预防与衰老或其他病理状况（如糖尿病）相关的肌肉损失方面具有广阔的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/de76b750b4e4/figs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/9f63931b832f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/082420cb08cc/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/e5511d00a2ad/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/680599238874/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/f8a5dd18c388/figs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/de76b750b4e4/figs2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/9f63931b832f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/082420cb08cc/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/e5511d00a2ad/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/680599238874/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/f8a5dd18c388/figs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edb/5766703/de76b750b4e4/figs2.jpg

相似文献

1
Black ginseng activates Akt signaling, thereby enhancing myoblast differentiation and myotube growth.黑参激活Akt信号通路，从而增强成肌细胞分化和肌管生长。
J Ginseng Res. 2018 Jan;42(1):116-121. doi: 10.1016/j.jgr.2017.08.009. Epub 2017 Sep 6.
2
Ginsenoside Rb1 and Rb2 upregulate Akt/mTOR signaling-mediated muscular hypertrophy and myoblast differentiation.人参皂苷Rb1和Rb2上调Akt/mTOR信号介导的肌肉肥大和成肌细胞分化。
J Ginseng Res. 2020 May;44(3):435-441. doi: 10.1016/j.jgr.2019.01.007. Epub 2019 Jan 31.
3
Ginsenoside Rg1 from enhances myoblast differentiation and myotube growth.来自[具体来源未提及]的人参皂苷Rg1可增强成肌细胞分化和肌管生长。
J Ginseng Res. 2017 Oct;41(4):608-614. doi: 10.1016/j.jgr.2017.05.006. Epub 2017 Jun 7.
4
The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy.胶原蛋白衍生的二肽羟脯氨酰甘氨酸可促进C2C12成肌细胞分化和肌管肥大。
Biochem Biophys Res Commun. 2016 Sep 23;478(3):1292-7. doi: 10.1016/j.bbrc.2016.08.114. Epub 2016 Aug 21.
5
Ginsenoside Rg3 upregulates myotube formation and mitochondrial function, thereby protecting myotube atrophy induced by tumor necrosis factor-alpha.人参皂苷 Rg3 通过上调肌管形成和线粒体功能，从而保护肿瘤坏死因子-α诱导的肌管萎缩。
J Ethnopharmacol. 2019 Oct 5;242:112054. doi: 10.1016/j.jep.2019.112054. Epub 2019 Jul 2.
6
Lactate Promotes Myoblast Differentiation and Myotube Hypertrophy via a Pathway Involving MyoD In Vitro and Enhances Muscle Regeneration In Vivo.乳酸通过涉及 MyoD 的途径在体外促进成肌细胞分化和肌管肥大，并增强体内肌肉再生。
Int J Mol Sci. 2018 Nov 19;19(11):3649. doi: 10.3390/ijms19113649.
7
Beef extract supplementation promotes myoblast proliferation and myotube growth in C2C12 cells.牛肉提取物补充剂促进 C2C12 细胞中的成肌细胞增殖和肌管生长。
Eur J Nutr. 2020 Dec;59(8):3735-3743. doi: 10.1007/s00394-020-02205-4. Epub 2020 Feb 25.
8
Ginsenoside Rg5 promotes muscle regeneration via p38MAPK and Akt/mTOR signaling.人参皂苷Rg5通过p38丝裂原活化蛋白激酶（p38MAPK）和蛋白激酶B/哺乳动物雷帕霉素靶蛋白（Akt/mTOR）信号通路促进肌肉再生。
J Ginseng Res. 2023 Nov;47(6):726-734. doi: 10.1016/j.jgr.2023.06.004. Epub 2023 Jun 24.
9
Diarylheptanoid 1-(4-hydroxyphenyl)-7-phenyl-(6E)-6-hepten-3-one enhances C2C12 myoblast differentiation by targeting membrane estrogen receptors and activates Akt-mTOR and p38 MAPK-NF-κB signaling axes.二芳基庚烷 1-(4- 羟基苯基)-7- 苯基-(6E)-6-庚烯-3-酮通过靶向膜雌激素受体增强 C2C12 成肌细胞分化，并激活 Akt-mTOR 和 p38 MAPK-NF-κB 信号通路。
J Nat Med. 2019 Sep;73(4):735-744. doi: 10.1007/s11418-019-01322-7. Epub 2019 May 13.
10
Glucosamine inhibits myoblast proliferation and differentiation, and stimulates myotube atrophy through distinct signal pathways.氨基葡萄糖通过不同的信号通路抑制成肌细胞增殖和分化，并刺激肌管萎缩。
J Nutr Biochem. 2025 Jan;135:109762. doi: 10.1016/j.jnutbio.2024.109762. Epub 2024 Sep 7.

引用本文的文献

1
Exploring the role of traditional Chinese medicine in sarcopenia: mechanisms and therapeutic advances.探索中医在肌肉减少症中的作用：机制与治疗进展
Front Pharmacol. 2025 Jun 30;16:1541373. doi: 10.3389/fphar.2025.1541373. eCollection 2025.
2
Black ginseng under forest as a natural antidepressant: insights into its active components and mechanisms.林下黑参作为一种天然抗抑郁剂：对其活性成分及作用机制的见解
Front Chem. 2025 May 30;13:1619060. doi: 10.3389/fchem.2025.1619060. eCollection 2025.
3
Therapeutic applications of ginseng for skeletal muscle-related disorder management.

本文引用的文献

1
Ameliorative Effects and Possible Molecular Mechanism of Action of Black Ginseng (Panax ginseng) on Acetaminophen-Mediated Liver Injury.黑参（人参）对乙酰氨基酚介导的肝损伤的改善作用及可能的分子作用机制
Molecules. 2017 Apr 21;22(4):664. doi: 10.3390/molecules22040664.
2
Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats.发酵黑参对顺铂诱导的大鼠肾毒性的预防作用。
J Ginseng Res. 2017 Apr;41(2):188-194. doi: 10.1016/j.jgr.2016.03.001. Epub 2016 Mar 8.
3
PKN2 and Cdo interact to activate AKT and promote myoblast differentiation.
人参在骨骼肌相关疾病管理中的治疗应用。
J Ginseng Res. 2024 Jan;48(1):12-19. doi: 10.1016/j.jgr.2023.06.003. Epub 2023 Jun 22.
4
Ginsenoside Rg5 promotes muscle regeneration via p38MAPK and Akt/mTOR signaling.人参皂苷Rg5通过p38丝裂原活化蛋白激酶（p38MAPK）和蛋白激酶B/哺乳动物雷帕霉素靶蛋白（Akt/mTOR）信号通路促进肌肉再生。
J Ginseng Res. 2023 Nov;47(6):726-734. doi: 10.1016/j.jgr.2023.06.004. Epub 2023 Jun 24.
5
Enhances Myotube Hypertrophy through PI3K-Mediated Akt/mTOR Signaling Phosphorylation.通过 PI3K 介导的 Akt/mTOR 信号通路磷酸化增强肌管肥大。
Nutrients. 2022 Apr 17;14(8):1670. doi: 10.3390/nu14081670.
6
Research Quality-Based Multivariate Modeling for Comparison of the Pharmacological Effects of Black and Red Ginseng.基于研究质量的多变量建模比较黑参和红参的药理作用。
Nutrients. 2020 Aug 26;12(9):2590. doi: 10.3390/nu12092590.
7
Ginsenoside Rb1 and Rb2 upregulate Akt/mTOR signaling-mediated muscular hypertrophy and myoblast differentiation.人参皂苷Rb1和Rb2上调Akt/mTOR信号介导的肌肉肥大和成肌细胞分化。
J Ginseng Res. 2020 May;44(3):435-441. doi: 10.1016/j.jgr.2019.01.007. Epub 2019 Jan 31.
8
Methylation determines the extracellular calcium sensitivity of the leak channel NALCN in hippocampal dentate granule cells.甲基化决定海马齿状颗粒细胞中漏通道 NALCN 的细胞外钙敏感性。
Exp Mol Med. 2019 Oct 10;51(10):1-14. doi: 10.1038/s12276-019-0325-0.
9
Black Ginseng and Its Saponins: Preparation, Phytochemistry and Pharmacological Effects.黑参及其皂苷：制备、植物化学和药理作用。
Molecules. 2019 May 14;24(10):1856. doi: 10.3390/molecules24101856.
10
PRMT7 methylates and suppresses GLI2 binding to SUFU thereby promoting its activation.PRMT7 通过甲基化修饰 SUFU 并抑制 GLI2 与其结合，从而促进 GLI2 的激活。
Cell Death Differ. 2020 Jan;27(1):15-28. doi: 10.1038/s41418-019-0334-5. Epub 2019 Apr 18.
PKN2与Cdo相互作用以激活AKT并促进成肌细胞分化。
Cell Death Dis. 2016 Oct 20;7(10):e2431. doi: 10.1038/cddis.2016.296.
4
Black ginseng extract exerts anti-hyperglycemic effect via modulation of glucose metabolism in liver and muscle.黑参提取物通过调节肝脏和肌肉中的葡萄糖代谢发挥抗高血糖作用。
J Ethnopharmacol. 2016 Aug 22;190:231-40. doi: 10.1016/j.jep.2016.05.060. Epub 2016 May 31.
5
Bakuchiol augments MyoD activation leading to enhanced myoblast differentiation.补骨脂酚增强MyoD激活，导致成肌细胞分化增强。
Chem Biol Interact. 2016 Mar 25;248:60-7. doi: 10.1016/j.cbi.2016.02.008. Epub 2016 Feb 18.
6
SMYD1 and G6PD modulation are critical events for miR-206-mediated differentiation of rhabdomyosarcoma.SMYD1和G6PD调节是miR-206介导的横纹肌肉瘤分化的关键事件。
Cell Cycle. 2015;14(9):1389-402. doi: 10.1080/15384101.2015.1005993.
7
Evaluating the validity of the Braden scale using longitudinal electronic medical records.使用纵向电子病历评估Braden量表的有效性。
Res Nurs Health. 2015 Apr;38(2):152-61. doi: 10.1002/nur.21642. Epub 2014 Dec 30.
8
Detoxifying effect of fermented black ginseng on H2O2-induced oxidative stress in HepG2 cells.发酵黑参对H2O2诱导的HepG2细胞氧化应激的解毒作用。
Int J Mol Med. 2014 Dec;34(6):1516-22. doi: 10.3892/ijmm.2014.1972. Epub 2014 Oct 16.
9
Muscle atrophy reversed by growth factor activation of satellite cells in a mouse muscle atrophy model.生长因子激活卫星细胞可逆转小鼠肌肉萎缩模型中的肌肉萎缩。
PLoS One. 2014 Jun 25;9(6):e100594. doi: 10.1371/journal.pone.0100594. eCollection 2014.
10
Signalling pathways regulating muscle mass in ageing skeletal muscle: the role of the IGF1-Akt-mTOR-FoxO pathway.调控衰老骨骼肌质量的信号通路：IGF1-Akt-mTOR-FoxO 通路的作用。
Biogerontology. 2013 Jun;14(3):303-23. doi: 10.1007/s10522-013-9432-9. Epub 2013 May 19.