Ismail Hammad, Rasheed Ammara, Haq Ihsan-Ul, Jafri Laila, Ullah Nazif, Dilshad Erum, Sajid Moniba, Mirza Bushra
Department of Biochemistry and Biotechnology, University of Gujrat, Gujrat 50700, Pakistan.
Department of Biochemistry, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Evid Based Complement Alternat Med. 2017;2017:7849501. doi: 10.1155/2017/7849501. Epub 2017 Nov 23.
Five medicinal plants of Pakistan were investigated for their antinociceptive, anti-inflammatory, antidepressant, and anticoagulant potential. Antinociceptive activity was estimated by hot plate and writhing assay. In hot plate assay, (52.2%) and (59.1%) showed moderate while (65.3%) displayed a significant reduction in pain. On the other hand, in writhing assay, (49.6%), (52.7%), and (62.0%) showed comparative less activity. In anti-inflammatory assays crude extracts showed significant edema inhibition in a dose dependent manner. In carrageenan assay, the highest activity was observed for (70.0%) followed by (66.7%) and (63.3%). Similar behavior was observed in histamine assay with percentage inhibitions of 74.3%, 60.4%, and 63.5%, respectively. Antidepressant activity was estimated by forced swim test and the most potent activity was revealed by with immobility time 2.2s (95.9%) followed by with immobility time 25.3s (53.4%). Moreover, the crude extracts of (74.6%), (73.8%), and (67.3%) showed good anticoagulant activity with coagulation times 86.9s, 84.3s, and 67.5s, respectively. Collectively, the results demonstrate that these five plants have rich medicinal constituents which can be further explored.
对巴基斯坦的五种药用植物进行了抗伤害感受、抗炎、抗抑郁和抗凝血潜力的研究。通过热板法和扭体试验评估抗伤害感受活性。在热板试验中，[植物名称1]（52.2%）和[植物名称2]（59.1%）表现出中度活性，而[植物名称3]（65.3%）显示出疼痛显著减轻。另一方面，在扭体试验中，[植物名称4]（49.6%）、[植物名称5]（52.7%）和[植物名称6]（62.0%）表现出相对较低的活性。在抗炎试验中，粗提物呈剂量依赖性地显著抑制水肿。在角叉菜胶试验中，[植物名称7]（70.0%）的活性最高，其次是[植物名称8]（66.7%）和[植物名称9]（63.3%）。在组胺试验中观察到类似的行为，抑制率分别为74.3%、60.4%和63.5%。通过强迫游泳试验评估抗抑郁活性，[植物名称10]表现出最强的活性，不动时间为2.2秒（95.9%），其次是[植物名称11]，不动时间为25.3秒（53.4%）。此外，[植物名称12]（74.6%）、[植物名称13]（73.8%）和[植物名称14]（67.3%）的粗提物表现出良好的抗凝血活性，凝血时间分别为86.9秒、84.3秒和67.5秒。总体而言，结果表明这五种植物含有丰富的药用成分，值得进一步探索。
