Hegde V L, Mahesh P A, Venkatesh Y P
Department of Biochemistry and Nutrition, CSIR - Central Food Technological Research Institute (CFTRI), Mysuru, Karnataka, India. Department of Radiation Oncology, The Houston Methodist Research Institute, Houston, TX 77030, U.S.A.
Allergy Asthma Associates, K. M. Puram, Mysuru, Karnataka, India.
Eur Ann Allergy Clin Immunol. 2018 Jan;50(1):10-18. doi: 10.23822/EurAnnACI.1764-1489.27.
The association between grass pollen sensitization and food allergy to tomato is of great interest. We report here, the first such study in Indian population. We investigated 246 allergic rhinitis / asthma patients by diagnostic case history and skin prick test (SPT); grass pollen mix, tomato extract and purified tomato profilin were used for SPT. Tomato profilin was purified by affinity chromatography, and analyzed by HPLC (95% purity) and SDS-PAGE (14 kDa). We observed that 38% of the patients had sensitization to both grass pollen and tomato fruit, of which 92% were sensitized to tomato profilin. Among patients with a history of food allergy to tomato fruit, the association was more pronounced (66%). Tomato profilin appears to be an important cross-sensitizing panallergen in respiratory allergic patients in the Indian subcontinent.
草花粉致敏与番茄食物过敏之间的关联备受关注。我们在此报告印度人群中的首例此类研究。我们通过诊断病史和皮肤点刺试验(SPT)对246例过敏性鼻炎/哮喘患者进行了调查;草花粉混合液、番茄提取物和纯化的番茄肌动蛋白结合蛋白用于SPT。番茄肌动蛋白结合蛋白通过亲和层析法纯化,并通过高效液相色谱法(纯度95%)和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(14 kDa)进行分析。我们观察到38%的患者对草花粉和番茄果实均致敏,其中92%对番茄肌动蛋白结合蛋白致敏。在有番茄果实食物过敏史的患者中,这种关联更为明显(66%)。番茄肌动蛋白结合蛋白似乎是印度次大陆呼吸道过敏患者中一种重要的交叉致敏泛过敏原。