Thonar E J, Lenz M E, Klintworth G K, Caterson B, Pachman L M, Glickman P, Katz R, Huff J, Kuettner K E
Arthritis Rheum. 1985 Dec;28(12):1367-76. doi: 10.1002/art.1780281209.
We have developed an enzyme-linked immunosorbent-inhibition assay which makes use of a monoclonal antibody specific for keratan sulfate to quantify keratan sulfate present as single chains in adult human serum. In adults hospitalized with conditions not thought to affect the turnover of keratan sulfate-containing tissues, the serum levels varied from individual to individual (53-1,009 ng/ml) but did not show significant differences with respect to age, sex, or disease category. There were no significant differences between the serum levels of adult hospitalized patients and those of nonhospitalized normal adults. In contrast, the concentration of keratan sulfate in the sera of children aged 5-12 was significantly higher. No keratan sulfate was detected in the sera of 3 adult patients with macular corneal dystrophy, an inherited disorder of the cornea. This may indicate that individuals with macular corneal dystrophy have no keratan sulfate-containing proteoglycans in their cartilage. Adult patients with osteoarthritis have significantly higher concentrations of circulating keratan sulfate. This suggests that the assay could prove valuable in monitoring increased cartilage catabolism in joint diseases.
我们开发了一种酶联免疫吸附抑制测定法，该方法利用一种对硫酸角质素具有特异性的单克隆抗体来定量测定成人血清中以单链形式存在的硫酸角质素。在因被认为不影响含硫酸角质素组织更新的疾病而住院的成年人中，血清水平因人而异（53 - 1009纳克/毫升），但在年龄、性别或疾病类别方面未显示出显著差异。住院成年患者的血清水平与未住院的正常成年人之间没有显著差异。相比之下，5 - 12岁儿童血清中的硫酸角质素浓度明显更高。在3例患有黄斑角膜营养不良（一种遗传性角膜疾病）的成年患者的血清中未检测到硫酸角质素。这可能表明患有黄斑角膜营养不良的个体软骨中不存在含硫酸角质素的蛋白聚糖。骨关节炎成年患者循环中的硫酸角质素浓度明显更高。这表明该测定法在监测关节疾病中软骨分解代谢增加方面可能具有重要价值。
