Institute for Genetics, Department of Biology, University of Cologne, Zuelpicher Str. 47a, 50674 Cologne, Germany.
Institute for Genetics, Department of Biology, University of Cologne, Zuelpicher Str. 47a, 50674 Cologne, Germany
Biochem Soc Trans. 2018 Feb 19;46(1):153-161. doi: 10.1042/BST20170059. Epub 2018 Jan 19.
During splicing, the exon junction complex (EJC) is deposited upstream of exon-exon boundaries. The EJC and its peripheral bound proteins play an essential role in mediating mRNA export, translation and turnover. However, the exact sequence of EJC assembly and the involved factors during splicing remain elusive. Recently published structures of the human C* spliceosome clarified the position of the EJC at this phase of splicing and have given insight into previously unidentified interactions between the EJC and spliceosomal proteins. Here, these new observations are presented and the significance for EJC assembly is discussed. Furthermore, the vast landscape of EJC interacting proteins and their manifold functions are described. Finally, the factors involved in EJC disassembly and recycling are recapitulated. This review aims to integrate structural, biochemical and physiological data to obtain a comprehensive picture of EJC components during the lifetime of the EJC.
在剪接过程中，外显子结合复合体（EJC）被沉积在exon-exon 边界的上游。EJC 及其外围结合蛋白在介导 mRNA 输出、翻译和周转中发挥着重要作用。然而，EJC 组装的确切顺序以及剪接过程中涉及的因素仍不清楚。最近发表的人类 C*剪接体结构阐明了 EJC 在这一剪接阶段的位置，并深入了解了 EJC 与剪接体蛋白之间以前未识别的相互作用。在这里，呈现了这些新的观察结果，并讨论了 EJC 组装的意义。此外，还描述了 EJC 相互作用蛋白的广阔景观及其多种功能。最后，综述了 EJC 解组装和再循环涉及的因素。本综述旨在整合结构、生化和生理数据，以获得 EJC 组件在 EJC 生命周期内的全面图景。
