Lacroix B, Kedinger M, Simon-Assmann P, Haffen K
Biol Cell. 1985;54(3):235-9. doi: 10.1111/j.1768-322x.1985.tb00399.x.
The aim of the present study was to test the morphological and functional maturation of recombinants composed of chick intestinal endoderms associated to different mesenchymal supports and their enzymatic response to glucocorticoids. For this purpose 5.5-day chick embryonic intestinal endoderm has been associated to 14-day fetal rat gut mesenchyme, to rat intestinal fibroblasts (6-day neonatal rat intramucosal fibroblasts) or to rat control fibroblasts, originating from 20-day fetal rat skin and lung and from 6-day neonatal rat intestinal muscle. The recombinants were grown as intracoelomic grafts either for 12 days or for 10 days plus 2 days in organ culture in the presence of dexamethasone. The data show that heterospecific recombinants achieve subnormal morphogenesis and enzymatic maturation. The organ culture experiments further reveal that sucrase activity is insensitive to dexamethasone in all types of recombinants whereas, alkaline phosphatase is highly stimulated over the levels present in the intestine developed in situ whatever the stromal support, except when this support is provided by rat gut mesenchyme. These results support the view that in the intestine the hormonal response is mediated by epithelial-mesenchymal interactions.
本研究的目的是测试由鸡肠道内胚层与不同间充质支持物组成的重组体的形态和功能成熟情况，以及它们对糖皮质激素的酶促反应。为此，将5.5天鸡胚的肠道内胚层与14天胎鼠肠道间充质、大鼠肠道成纤维细胞（6天新生大鼠黏膜内成纤维细胞）或大鼠对照成纤维细胞相结合，对照成纤维细胞来源于20天胎鼠的皮肤和肺以及6天新生大鼠的肠道肌肉。重组体作为体腔内移植物培养12天，或在存在地塞米松的情况下在器官培养中培养10天加2天。数据表明，异种特异性重组体的形态发生和酶成熟低于正常水平。器官培养实验进一步表明，在所有类型的重组体中，蔗糖酶活性对地塞米松不敏感，而无论基质支持物如何，碱性磷酸酶的活性都比原位发育的肠道中的水平受到高度刺激，除非这种支持物由大鼠肠道间充质提供。这些结果支持了这样一种观点，即在肠道中，激素反应是由上皮-间充质相互作用介导的。
