Haffen K, Kedinger M, Simon P M, Raul F
Differentiation. 1981;18(2):97-103. doi: 10.1111/j.1432-0436.1981.tb01109.x.
The aim of the present study was to investigate the mesenchymal influence on cultured epithelioid cells originating from an already differentiated intestine. Epithelioid cell cultures of 6-day-old suckling rat intestine were established by sequential trypsinizations of the mucosa. Embryonic intestinal monolayers of quail cells (13 days) were used as control because of their natural cell marker. Six to thirty days after plating, both types of epithelioid cells were associated in heterospecific combination with 5 1/2-day-old chick embryonic small intestinal mesenchyme, after removal of the endoderm by collagenase treatment. In order to test the differentiation capabilities of the associations, they were grafted for 10-12 days into 3-day-old chick embryos. The results show that in such an in vivo culture system, the chimeric associations gave rise to well differentiated intestinal structures indicating that the epithelioid cell cultures derived from late embryonic or neonatal intestine will go through organotypic differentiation when recombined with an appropriate mesenchyme.
本研究的目的是探讨间充质对源自已分化肠道的培养上皮样细胞的影响。通过对黏膜进行连续胰蛋白酶消化，建立了6日龄乳鼠肠道的上皮样细胞培养物。鹌鹑细胞（13日龄）的胚胎肠单层用作对照，因为它们有天然的细胞标记物。接种后6至30天，通过胶原酶处理去除内胚层后，两种类型的上皮样细胞都与5.5日龄鸡胚小肠间充质进行异种特异性组合。为了测试这些组合的分化能力，将它们移植到3日龄鸡胚中10至12天。结果表明，在这样的体内培养系统中，嵌合组合产生了分化良好的肠道结构，这表明源自晚期胚胎或新生肠道的上皮样细胞培养物在与适当的间充质重组时将经历器官型分化。
