University of Cambridge, Plant Sciences, Cambridge, CB2 3EA, United Kingdom.
Centro de Investigaciones Biotecnologicas del Ecuador, Facultad de Ciencias de la Vida, Escuela Superior Politecnica del Litoral, ESPOL, Guayaquil, Ecuador.
Sci Rep. 2018 Jan 19;8(1):1173. doi: 10.1038/s41598-018-19607-4.
Maize chlorotic mottle virus has been rapidly spreading around the globe over the past decade. The interactions of maize chlorotic mottle virus with Potyviridae viruses causes an aggressive synergistic viral condition - maize lethal necrosis, which can cause total yield loss. Maize production in sub-Saharan Africa, where it is the most important cereal, is threatened by the arrival of maize lethal necrosis. We obtained maize chlorotic mottle virus genome sequences from across East Africa and for the first time from Ecuador and Hawaii, and constructed a phylogeny which highlights the similarity of Chinese to African isolates, and Ecuadorian to Hawaiian isolates. We used a measure of clustering, the adjusted Rand index, to extract region-specific SNPs and coding variation that can be used for diagnostics. The population genetics analysis we performed shows that the majority of sequence diversity is partitioned between populations, with diversity extremely low within China and East Africa.
过去十年间，玉米条纹病毒在全球范围内迅速传播。玉米条纹病毒与马铃薯 Y 病毒科病毒的相互作用导致了一种具有侵略性的协同病毒状况——玉米坏死性致死，这可能导致总产量损失。玉米是撒哈拉以南非洲最重要的谷物，玉米坏死性致死的出现威胁到了该地区的玉米生产。我们从东非各地获得了玉米条纹病毒基因组序列，这是首次从厄瓜多尔和夏威夷获得该病毒序列，并构建了一个系统发育树，该树突出了中国和非洲分离株以及厄瓜多尔和夏威夷分离株的相似性。我们使用聚类度量——调整后的 Rand 指数，提取了可用于诊断的特定区域 SNP 和编码变异。我们进行的种群遗传分析表明，大多数序列多样性在种群之间划分，中国和东非内部的多样性极低。
