School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, China.
Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing, 100084, China.
Carbohydr Polym. 2018 Mar 15;184:445-452. doi: 10.1016/j.carbpol.2018.01.005. Epub 2018 Jan 2.
It is of great significance to construct multifunctional nanosystems for simultaneous imaging and therapy of cancer cells. Herein, PEGylated chitosan nanoparticles with embedded bismuth sulfide were facilely fabricated via reverse-microemulsion method for fluorescent imaging and photothermal therapy of HepG2 cells. The obtained BSA-BiS-CG-PEG nanospheres revealed dual-wavelength fluorescence, which were spectrally isolated from the bioautofluorescence. Moreover, they demonstrated remarkable photothermal conversion efficiency and stability. Importantly, these small BSA-BiS-CG-PEG nanoparticles shown a zeta potential of + 42.3 mV, which could rapidly get into HepG2 cells and locate in the cytoplasm and nuclei of cells. Based on their excellent photothermal effect and high cellular uptake, BSA-BiS-CG-PEG nanoparticles could efficiently kill HepG2 cells under an 808 nm laser irradiation. This construction strategy can be used for preparation of fluorescent chitosan nanoparticles with other therapeutic agents embedded, which would provide a versatile platform for dual-wavelength fluorescent imaging guided therapy of cancer.
构建用于同时对癌细胞进行成像和治疗的多功能纳米系统具有重要意义。本文通过反微乳液法制备了壳聚糖纳米粒子，该纳米粒子通过嵌入硫化铋来实现对 HepG2 细胞的荧光成像和光热治疗。所得的 BSA-BiS-CG-PEG 纳米球具有双波长荧光，其光谱与生物自发荧光分离。此外，它们还表现出显著的光热转换效率和稳定性。重要的是，这些小的 BSA-BiS-CG-PEG 纳米颗粒的zeta 电位为+42.3 mV，能够快速进入 HepG2 细胞，并定位在细胞质和细胞核中。基于其优异的光热效应和高细胞摄取率，BSA-BiS-CG-PEG 纳米颗粒在 808nm 激光照射下可以有效地杀死 HepG2 细胞。这种构建策略可用于制备嵌入其他治疗剂的荧光壳聚糖纳米颗粒，为双波长荧光成像引导癌症治疗提供了一个通用平台。
