Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz, Banacha12/16, 90-237 Lodz, Poland; Department of General Biophysics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Department of General Biophysics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
J Inorg Biochem. 2018 Apr;181:18-27. doi: 10.1016/j.jinorgbio.2018.01.001. Epub 2018 Jan 12.
Dendrimers, which are considered as one of the most promising tools in the field of nanobiotechnology due to their structural organization, showed a great potential in gene therapy, drug delivery, medical imaging and as antimicrobial and antiviral agents. This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into the cancer cells. Moreover, dendrimers can protect siRNA against nuclease degradation. Nevertheless, further research need to be performed to examine the therapeutic potential of ruthenium dendrimers as well as dendrimers complexed with siRNA and anticancer drugs towards cancer cells.
树状大分子，由于其结构组织，被认为是纳米生物技术领域中最有前途的工具之一，在基因治疗、药物传递、医学成像以及作为抗菌和抗病毒剂方面显示出巨大的潜力。本文致力于研究含有钌的新型基于碳硅烷的金属树状大分子与抗癌小干扰 RNA（siRNA）之间的相互作用。通过透射电子显微镜、圆二色性和荧光评估抗癌 siRNA 与 Ru 基碳硅烷树状大分子之间形成复合物的情况。通过动态光散射测定树枝状聚合物的 ζ 电位和粒径。使用 HL-60 细胞估计树枝状聚合物的内化。结果表明，与抗癌 siRNA 结合的钌树状大分子具有将 siRNA 作为非病毒载体递送入癌细胞的能力。此外，树突状聚合物可以保护 siRNA 免受核酸酶的降解。然而，需要进一步的研究来检查钌树突状聚合物以及与 siRNA 和抗癌药物复合的树突状聚合物对癌细胞的治疗潜力。
