Departamento de Engenharia Química, Universidade Regional de Blumenau, Blumenau, Santa Catarina, 89030-000, Brazil.
Departamento de Química, Universidade Federal do Paraná, Cx. P. 19081 Centro Politécnico, Curitiba, Paraná, 81531-980, Brazil.
Bioprocess Biosyst Eng. 2018 Apr;41(4):573-583. doi: 10.1007/s00449-018-1892-5. Epub 2018 Jan 20.
A fermented solid containing lipases was produced by solid-state fermentation of Rhizopus microsporus on sugarcane bagasse enriched with urea, soybean oil, and a mineral solution. The dry fermented solid produced using R. microsporus (RMFS) was used to catalyze the synthesis of alkyl-esters by esterification in a solvent-free system containing ethanol and oleic acid (as a model system) or a mixture of fatty acids obtained from the physical hydrolysis of soybean soapstock acid oil (FA-SSAO) in subcritical water. The conversions were 93.5 and 84.1%, for oleic acid and FA-SSAO, respectively, at 48 h and 40 °C, at a molar ratio (MR) of ethanol to fatty acid of 5:1. A further increase in the MR to 10:1 improved the production of ethylic-esters, giving conversions at 48 h of 98 and 86% for oleic acid and FA-SSAO, respectively. The results obtained in this work foster further studies on scaling-up of an environmentally friendly process to produce biofuels.
一种含有脂肪酶的发酵固体是通过在富含尿素、大豆油和矿物质溶液的甘蔗渣上进行根霉属小孢霉的固态发酵生产的。使用根霉属小孢霉（RMFS）生产的干燥发酵固体用于在无溶剂系统中通过酯化催化合成烷基酯，该系统含有乙醇和油酸（作为模型系统）或从物理水解大豆皂脚酸化油（FA-SSAO）中获得的脂肪酸混合物在亚临界水中。在 48 小时和 40°C 下，摩尔比（MR）为乙醇与脂肪酸 5:1 时，油酸和 FA-SSAO 的转化率分别为 93.5%和 84.1%。进一步将 MR 增加到 10:1 提高了乙酯的产量，在 48 小时时油酸和 FA-SSAO 的转化率分别为 98%和 86%。这项工作的结果促进了进一步研究将环保工艺放大生产生物燃料。
