Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, SE-97187, Luleå, Sweden.
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT-R), Roorkee, Uttarakhand, 247667, India.
Environ Sci Pollut Res Int. 2018 Nov;25(33):33443-33454. doi: 10.1007/s11356-018-3183-8. Epub 2018 Sep 28.
Biodiesel production from vegetable oils is not sustainable and economical due to the food crisis worldwide. The development of a cost-effective non-edible feedstock is essential. In this study, we proposed to use aquatic oomycetes for microbial oils, which are cellulolytic fungus-like filamentous eukaryotic microorganisms, commonly known as water molds. They differ from true fungi as cellulose is present in their cell wall and chitin is absent. They show parasitic as well as saprophytic nature and have great potential to utilize decaying animal and plant debris in freshwater habitats. To study the triacylglycerol (TAG) accumulation in the aquatic oomycetes, the isolated water mold Achlya diffusa was cultivated under semi-solid-state conditions on waste sugarcane bagasse, which was compared with the cultivation in Czapek (DOX) medium. A. diffusa grown on waste sugarcane bagasse showed large lipid droplets in its cellular compartment and synthesized 124.03 ± 1.93 mg/gds cell dry weight with 50.26 ± 1.76% w/w lipid content. The cell dry weight and lipid content of this water mold decreased to 89.54 ± 1.21 mg/gds and 38.82% w/w, respectively, when cultivated on standard medium Czapek-Dox agar (CDA). For the fatty acid profile of A. diffusa grown in sugarcane bagasse and CDA, in situ transesterification (IST) and indirect transesterification (IDT) approaches were evaluated. The lipid profile of this mold revealed the presence of C, C, C, C, C, C, C, and C fatty acids, which is similar to vegetable oils. The biodiesel properties of the lipids obtained from A. diffusa satisfied the limits as determined by international standards ASTM-D6751 and EN-14214 demonstrating its suitability as a fuel for diesel engines.
由于全球范围内的粮食危机，从植物油中生产生物柴油既不可持续也不经济。开发具有成本效益的非食用原料至关重要。在这项研究中，我们提议使用水生卵菌门微生物油脂，它是一种纤维素分解菌样丝状真核微生物，通常被称为水霉菌。它们与真正的真菌不同，因为纤维素存在于它们的细胞壁中，而几丁质不存在。它们表现出寄生和腐生的性质，并且具有利用淡水生境中腐烂的动植物残骸的巨大潜力。为了研究水生卵菌门的三酰基甘油（TAG）积累，在半固态条件下，在废甘蔗渣上培养分离出的水霉菌 Achlya diffusa，并与在 Czapek (DOX) 培养基中的培养进行比较。在废甘蔗渣上生长的 A. diffusa 在其细胞隔室中显示出大的脂滴，并以 50.26% w/w 的脂质含量合成了 124.03±1.93 mg/gds 细胞干重。当在标准培养基 Czapek-Dox 琼脂（CDA）上培养时，这种水霉菌的细胞干重和脂质含量分别降至 89.54±1.21 mg/gds 和 38.82% w/w。对于在甘蔗渣和 CDA 中生长的 A. diffusa 的脂肪酸图谱，评估了原位酯交换（IST）和间接酯交换（IDT）方法。该模具的脂质图谱显示存在 C、C、C、C、C、C、C 和 C 脂肪酸，这与植物油相似。从 A. diffusa 获得的脂质的生物柴油特性符合 ASTM-D6751 和 EN-14214 国际标准规定的限值，证明其适合用作柴油发动机燃料。
