Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
Sci Total Environ. 2018 Jun 1;626:264-273. doi: 10.1016/j.scitotenv.2018.01.074. Epub 2018 Feb 19.
The objective of this study was to determine the influence of Tanfloc on Microcystis aeruginosa cell integrity, microcystin-LR (MC-LR), and proteins during flocculation and floc storage. The effects of Tanfloc addition, stirring, and floc storage time were considered to minimize cell damage and the release of MC-LR and proteins. Optimal flocculation conditions (Tanfloc dosage 10.42 mg L, rapid agitation for 0.36 min at 568.88 rpm and slow agitation for 14.14 min at 12.1 rpm) were obtained using the response surface methodology. Up to 98.9% of the M. aeruginosa cells were removed intact at low Tanfloc dosage. During floc storage, Tanfloc initially protected the cells. After 8 d, large-scale cell lysis occurred because Tanfloc had substantially decomposed. Nevertheless, Tanfloc also extended the extracellular MC-LR and protein release time to 8 d. This delay ensured adequate time to decontaminate sludge containing the algae, thereby reducing the risk of secondary pollution. In addition, the low cost of Tanfloc facilitates its widespread application in the management of harmful algal blooms.
本研究旨在探讨 Tanfloc 对铜绿微囊藻细胞完整性、微囊藻毒素-LR（MC-LR）和蛋白质的影响。考虑到 Tanfloc 的添加、搅拌和絮体储存时间的影响，以尽量减少细胞损伤、MC-LR 和蛋白质的释放。采用响应面法得到最佳的絮凝条件（Tanfloc 剂量 10.42mg/L，快速搅拌 0.36min，转速 568.88rpm，慢速搅拌 14.14min，转速 12.1rpm）。在低 Tanfloc 剂量下，高达 98.9%的铜绿微囊藻细胞完整去除。在絮体储存过程中，Tanfloc 最初保护了细胞。8d 后，由于 Tanfloc 大量分解，发生了大规模的细胞裂解。然而，Tanfloc 也将胞外 MC-LR 和蛋白质的释放时间延长至 8d。这种延迟确保了有足够的时间来处理含有藻类的污泥，从而降低了二次污染的风险。此外，Tanfloc 的低成本有利于其在有害藻类水华管理中的广泛应用。
