Kolta M G, Soliman K F, Williams B B
Horm Res. 1986;23(2):112-21. doi: 10.1159/000180298.
The effect of 5-hydroxytryptamine (5-HT) alteration on brain dopamine (DA), norepinephrine (NE), beta-endorphin (beta E) and immunoreactive insulin (IRI) was studied in Sprague-Dawley diabetic and control rats. Diabetes was induced using alloxan (45 mg/kg), 15 days prior to sacrificing. Both control and diabetic animals were treated with either p-chlorophenylalanine (PCPA, 300 mg/kg) 3 days prior to sacrificing or fluoxetine (10 mg/kg) twice daily for 3 days. PCPA treatment significantly decreased brain content of 5-HT and 5-hydroxyindole acetic acid (5-HIAA) while it caused significant increase and decrease in brain beta E and insulin levels, respectively, in both normal and diabetic rat. Meanwhile, the administration of fluoxetine resulted in significant increase in brain content of 5-HT, DA, NE and insulin but significant decline of beta E in diabetic and saline control rats. The results of this experiment indicate that 5-HT may be regulating both beta E and insulin regardless of the availability of pancreatic insulin.
在斯普拉格-道利糖尿病大鼠和对照大鼠中研究了5-羟色胺（5-HT）改变对脑多巴胺（DA）、去甲肾上腺素（NE）、β-内啡肽（βE）和免疫反应性胰岛素（IRI）的影响。在处死前15天，用四氧嘧啶（45mg/kg）诱导糖尿病。在处死前3天，对照动物和糖尿病动物均用对氯苯丙氨酸（PCPA，300mg/kg）治疗，或每天两次给予氟西汀（10mg/kg），持续3天。PCPA治疗显著降低了脑5-HT和5-羟吲哚乙酸（5-HIAA）含量，而在正常和糖尿病大鼠中，它分别导致脑βE和胰岛素水平显著升高和降低。同时，给予氟西汀导致糖尿病大鼠和生理盐水对照大鼠脑5-HT、DA、NE和胰岛素含量显著增加，但βE显著下降。本实验结果表明，无论胰腺胰岛素是否可用，5-HT可能同时调节βE和胰岛素。
